E1A基因对裸鼠移植瘤化疗增敏的实验研究

目的 探讨E1A基因对人宫颈癌细胞裸鼠移植瘤化疗的增敏作用及其相关作用机制.方法 通过E1A基因对裸鼠移植瘤化疗增敏实验,观察E1A基因对裸鼠移植瘤化疗敏感性的影响;采用RT-PCR鉴定E1A在肿瘤细胞中的表达及E1A转染后对HER-2/neu基因表达的影响.结果 E1A基因化疗增敏实验结果显示:E1A基因组裸鼠肿瘤体积缩小最明显,肿瘤重量明显小于对照组及空载体组(均P＜0.05);而后两组差异无显著性(P＞0.05).RT-PCR结果显示:E1A基因在宫颈癌细胞中能稳定表达并且显著降低HER-2/neu在宫颈癌细胞中的表达水平.结论 E1A基因能够显著增加紫杉醇对裸鼠移植瘤的药物敏感性.该作用机制可能与E1A基因降低HER-2/neu蛋白的表达有关.

Effect of E1A gene on the chemosensitivity to transplantation tumor in nude mice

Objective] This study is to investigate the chemosensitivity of E1A gene on human cervical carcinoma xenografts in nude mice and the correlative mechanism . Methods] Chemosensitivity of E1A gene on human cervical carcinoma xenografts in nude mice was observed by the experiment imitating chemotherapy on nude mice with E1A gene. The expression of E1A gene and its effect on HER-2/neu expression was detected by RT-PCR. Results] The experiment imitating chemotherapy on nude mice with E1A gene showed that the tumor volume in E1A group deflated most obviously, the tumor quality was evidently lower than control group and blank-vector group (P <0.05); and there is no statistical difference between the last two groups (P >0.05). RT-PCR demonstrated that E1A gene had a stable transfection in Hela cells and obviously down-regulated HER-2/neu expression. Comclusion] E1A gene can effectively enhance the chemosensitivity of human cervical carcinoma xenografts in nude mice to paclitaxel and the mechanism of action may relate to the down-regulation of the HER-2/neu expression by E1A gene .