| ELISpot技术的研究进展及临床应用 |
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| 引用本文： | 徐建青. ELISpot技术的研究进展及临床应用[J]. 中华检验医学杂志, 2010, 33(8). DOI: 10.3760/cma.j.issn.1009-9158.2010.08.001 |
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| 作者姓名： | 徐建青 |
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| 作者单位： | 上海市公共卫生临床中心复旦大学生物医学研究院,201508 |
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| 基金项目： | 国家艾滋病和病毒性肝炎等重大传染病防治科技专项资助课题，上海市科委中加合作项目 |
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| 摘 要： | 自ELISpot技术问世以来,由于其独特的优点,在基础与临床研究中的应用越来越广泛.目前,ELISpot技术为抗原表位筛查、抗原特异性T淋巴细胞定量、T淋巴细胞受体功能亲和力研究以及疫苗效果评价等提供了极其重要的技术平台;其在基础研究领域广泛应用的同时,ELISpot技术在临床中的应用也逐渐受到重视.现在,ELISpot技术正在疾病诊断与鉴别诊断、患者免疫状态和治疗效果评价以及疾病预后判断等方面发挥着重要作用.随着ELISpot技术的改进,相信ELISpot技术将为人类的健康事业做出更大的贡献.
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| 关 键 词： | 酶联免疫斑点技术 表位筛查 特异性T淋巴细胞 诊断 鉴别诊断 |
Research advance and clinical application of ELISpot assay |
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| XU Jian-qing. Research advance and clinical application of ELISpot assay[J]. Chinese Journal of Laboratory Medicine, 2010, 33(8). DOI: 10.3760/cma.j.issn.1009-9158.2010.08.001 |
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| Authors: | XU Jian-qing |
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| Abstract: | ELISpot assay has been widely used in basic and clinical research with its unique advantages. ELISpot is highly efficient in epitope screening, quantification of the epitope specific T cells, determining the TCR affinity and evaluating vaccine efficacy. In addition, ELISpot has been increasingly employed in diagnosis, differentiating diagnosis, evaluating patients' immune state, treatment efficacy, and evaluation of the prognosis. Overall, with the further improvement of this assay, ELISpot will play a critical role in the development of human health. |
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| Keywords: | Enzyme-linked immunospot Epitope screening T cell affinity Diagnosis Differentiating diagnosis |
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