5-羟色胺在大鼠实验性脑震荡脑组织中的表达

目的 探讨5-羟色胺在大鼠实验性脑震荡脑组织中的表达及其意义。方法 采用Wistar雄性二级大鼠复制脑震荡动物模型。于伤后1，3，7，14及30d取脑组织，经免疫组化等技术研究5-羟色胺在脑震荡发生发展中的变化规律。结果 100g组见典型脑震肠的临床表现，其病理改变为脑血管收缩与扩张，脑组织淤血，水肿，神经元变性，坏死，尼氏体减少甚至消失，5-羟色胺于伤后1d表达增强，3d达高峰，7d后开始减少，而于14d后逐渐恢复至正常水平。阳性染色见于大脑，海马，丘脑及小脑神经元胞浆内。结论 脑震荡以血液循环障碍和实质细胞变性，坏死为主要病理改变；5-羟色胺参与脑震荡发生时脑组织损伤的病理过程，可能是脑震荡时脑损伤的重要原因之一。

The expression of 5-hydroxytryptamine (5-HT) in rat concussive brain tissue

Objective To investigate the changes and significance of 5-hydroxytryptamine (5-HT) in rat cerebral concussion. Methods Eighty Wistar male rats were used for the animal model of cerebral concussion, and were sacrificed on 1,3,7,14 and 30 d after injury and the brain tissues were taken off. The expression of 5-HT in the course of cerebral concussion was examined by means of immunohistochemistry. Results The symptomatic appearances of typical brain concussion were observed in 100 g group. The pathologic changes were the cerebral vascular constriction and dilatation, brain congestion and edema. In addition, neuronal degeneration and necrosis were also observed. Nissl's bodies were dramatically diminished, even disappeared. The 5-HT expression was increased at 1 d, peaked at 3 d, turned down at 7 d and regressed to normal level at 14 d. The positive staining was seen in the cytoplasm of neurons in cortex, hippocampus, thalamus and cerebellum. Conclusion The main pathologic changes of cerebral concussion are blood circulatory disorder and neuronal degeneration and necrosis. The data suggest that 5-HT is involved in the process of brain concussion and may contribute to brain injury.