五味子乙素防护晶状体氧化损伤的体外实验研究

 目的探讨五味子乙素(Sch B)对实验性晶状体氧化损伤的防护作用。方法采用Fenton反应复制兔晶状体氧化损伤模型，以吡诺克辛钠滴眼液(PS)为对照，测定晶状体SP，SOD，GSH-Px，GSH，VitC及MDA的含量，观察终浓度0.5 mmol·L^-1Sch B对晶状体氧化损伤的防护作用。结果Sch B组与Fenton对照组比较，Sch B可显著减少Fenton反应中SP的丢失，显著提高SOD和GSH-Px的活性及GSH和Vit C的水平，并降低MDA含量。Sch B组与PS组比较，Sch B组的SOD活性、GSH含量和Vit C水平分别是PS组的1.66倍，2.58倍和2.36倍，MDA含量比PS组下降24%。上述各项指标均有显著性差异(P＜0.01)。结论 Sch B对Fenton反应中晶状体的氧化损伤有明显的防护作用，且优于吡诺克辛钠滴眼液。

An experimental study on protection of schisandrin B against oxidative damages of lens in vitro

OBJECTIVE To investigate the protection role of schiandrin B against experimental oxidative damages of lens.METHODS 20 New Zealand rabbits (40 eyes) were divided into four groups:(1)control group,(2)Fenton group (Fenton),(3)pirenoxine sodium group (PS),(4)0.5 mmol·L^-1schisandrin B group (Sch B).All fresh lenses except the control group were bathed in Fenton reaction system consisting of H₂O₂and FeCl₃as a model of oxidative damages of lens.Meanwhile PS and Sch B were added into the reaction system in PS and Sch B groups respectively.Lenses were incubated in CO₂incubator for 24 hours.Then soluble protein(SP),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),glutathione (GSH),Vitamin C (Vit C) and Malondiaoldehyde (MDA) in homogenized lenses were measured.RESULTS (1)Sch B group Compared with Fenton group:SP of lens in Sch B group was significantly higher than that in Fenton reaction system (P＜0.01);The activities of SOD and GSH-Px and the levels of GSH and Vit C in the lens of Sch B group increased;MDA decreased.(2)Sch B group Compared with PS group:SOD activity in Sch B group was 1.66 times the level of PS group;GSH in Sch B group was 2.58 times the level of PS group;Vit C,2.36 times;MDA decreased 24%(P＜0.01).CONCLUSION The findings showed that Sch B protected lens against oxidative damages in Fenton reaction system remarkably,and the anti oxidative role of Sch B was better than PS.The results provided scientific basis for using Sch B as a potential drug to prevent and treat cataract.