腺样囊性癌在纤维连接蛋白中趋化功能变化及其浸润机制

目的 通过腺样囊性癌（ａｄｅｎｏｉｄ ｃｙｓｔｉｃ ｃａｒｃｉｎｏｍａ，ＡＣＣ）在纤维连接蛋白（ｆｉｂｒｏｎｅｃｔｉｎ，ＦＮ）中的固相趋化功能变化探讨其浸润机制。方法 用Ｂｏｙｄｅｎ小室法和流式细胞术等测定了３种ＡＣＣ细胞对ＦＮ的趋化反应和细胞表面的受体变化。结果 ３种ＡＣＣ均比鳞状细胞癌（ｓｑｕａｍｏｕｓ ｃｅｌｌ ｃａｒｃｉｎｏｍａ，ＳＣＣ）趋化功能强；ＡＣＣ和ＳＣＣ细胞表面α２、α３、α６和β１受体亚单位水平高，且ＡＣＣ３α５也高；受体阻断可见：ＦＮ中阻断ＡＣＣ的α５、β１受体亚单位趋化功能呈高度抑制，阻断α４呈中度抑制，ＳＣＣ则阻断α１、α４和β１呈强抑制。结论 ＦＮ中ＡＣＣ比ＳＣＣ侵袭力强；各种细胞不同受体在ＦＮ中趋化反应的作用有一定规律。

Studies on infiltration mechanism of adenoid cystic carcinoma: haptotaxis function assay of fibronectin

OBJECTIVE: To investigate the invasion mechanism of adenoid cystic carcinoma (ACC) by ACC haptotaxis changes in fibronectin (FN). METHODS: Three strains of ACC cells were used. The haptotaxis changes in FN, and receptor changes in the cell membrane surface were determined. RESULTS: All three strains of ACC cells had remarkably greater migration ability than SCC cell. High level of alpha 2, alpha 3, alpha 6 and beta 1 receptor subunit types was found in the cell surface of ACC and SCC; and high level of alpha 5 in ACC3. High inhibition of haptotaxis reaction was found after blocked alpha 5 and beta 1 receptor subunit in ACC and a middle inhibition after blocked alpha 4. In SCC more strong inhibition was found after blocked alpha 1, alpha 4 and beta 1. CONCLUSIONS: This study indicates that ACC cells have greater invasion ability than SCC cells in FN and haptotaxis reaction of different receptor of tested cells in FN have some feature pattern.