氨茶碱对哮喘小鼠气道树突状细胞作用的研究

目的 研究氨茶碱对哮喘小鼠气道树突状细胞(DC)的密度和分布的影响。方法50只BALB/c小鼠随机分为正常对照组、哮喘对照组、低浓度氨茶碱组、中浓度氨茶碱组和高浓度氨茶碱组,每组10只。以卵蛋白腹腔注射与雾化吸入诱发BALB/c小鼠哮喘发作,末次激发24h后,采用免疫组织化学染色技术(DC标记物为NLDC-145)及计算机图像分析方法,观察哮喘小鼠在腹腔注射不同剂量氨茶碱1周后气道树突状细胞的分布和密度的改变。结果 正常小鼠NLDC-145~+树突状细胞主要分布于气管、支气管、肺泡、脏层胸膜及气管周围相关淋巴组织、血管周围,形成防御性DC网络。哮喘对照组小鼠气道NLDC-145~+DC密度为(1720±130)个/mm~2,显著高于正常对照组(600±200)个/mm~2(P<0.02);经氨茶碱治疗后,低浓度组气道DC密度为(824±35)个/mm~2,较哮喘对照组明显下降(P<0.01);中浓度组为(1582±150)个/mm~2,高浓度组为(1684±167)个/mm~2,与哮喘对照组比较无显著差异(P>0.05);哮喘对照组及低浓度氨茶碱组气道DC的分布与正常对照组相比无明显变化。结论 树突状细胞在哮喘小鼠气道中明显增多,低浓度氨茶碱可减少气道DC密度,但不影响其分布。

Effect of aminophylline on the distribution and density of airway intraepithelial dendritic cells in murine models

Objective To investigate the effect of aminophylline-treatment on the distribution and density of airway intraepithelial dendritic cells in murine models.Methods 50 BALB/c mouse were randomly divided into five groups: control group, asthmatic group,aminophylline -treatment group(low,mid-dle^igh concentrations) .The animal model of asthma was established by OVA sensitizing-challenging. 24 h after the last challenge, the pulmonary and tracheal samples were performed. Immunohistochemistry was used to detect DC, furthermore, transmission electron microscope and computer image analysis were applied to analyse the morphology of EC.Results After OVA challenge, the whole amounts of dendritic cells in tracheal, bronchus and alveolar were revealed by horseradish peroxidase staining using the NLDC-145 monoclonal antibody. There were more NLDC-145 + DC in the bronchial mucosa of asthmatic mouse compared with healthy controls(P<0.02) .After seven days of treament with aminophylline, NLDC-145+ DC in low concentration group were decreased than compared with those in asthmatic group(P< 0.01) , while in middle and high concentration group, there were no obvious change (P>0.05). Conclusion It is suggested that dendritic cells are involved in inflammation of airway of asthmatic murine and that low concentration aminophylline may down-regulate the numbers of dendritic cells.