| 5-氟尿嘧啶诱导肾癌细胞凋亡对Fas/FasL途径依赖性的研究 |
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| 引用本文： | 吴国英,李黔生,聂志林,李彦锋,靳风烁.5-氟尿嘧啶诱导肾癌细胞凋亡对Fas/FasL途径依赖性的研究[J].第三军医大学学报,2009,31(13). |
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| 作者姓名： | 吴国英 李黔生 聂志林 李彦锋 靳风烁 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所泌尿外科,重庆,400042 |
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| 摘 要： | 目的 探讨Fas/FasL凋亡途径在5-氟尿嘧啶(5-FU)诱导的肾癌细胞凋亡中的作用.方法 运用Western blot及TUNEL、MTT技术,检测肾癌细胞株786-0在化疗药物5-FU作用下Fas、FasL的表达变化,分析5-FU、抗Fas单抗CH11、中和性抗FasL单抗NOK-2对癌细胞凋亡与增殖活性的影响.结果 Fas、FasL蛋白产物在786-0中均有表达,不同浓度5-Fu作用后,中等剂量5-FU 70μg/ml作用肾癌细胞株48 h时Fas、FasL的表达最显著;786-0在化疗药物5-FU作用下,细胞增殖活性显著降低,细胞凋亡指数显著增加;抗Fas单抗CH11作用于786-0后,细胞凋亡指数显著增加,增殖活性显著降低;CH11与5-FU共同作用于786-0细胞可显著抑制细胞增殖,诱导细胞凋亡;抗FasL单抗NOK-2阻断786-0 FasL与Fas相互作用后,细胞的凋亡指数及增殖活性均无显著变化.结论 5-FU诱导肾癌细胞凋亡可能不依赖于Fas/FasL凋亡途径.
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| 关 键 词： | 肾癌 耐药 5-氟尿嘧啶 凋亡 |
Dependence of 5-Fluorouracil inducing cell apoptosis of renal cell carcinoma on Fas/FasL pathway |
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| WU Guo-ying,LI Qian-sheng,NIE Zhi-lin,LI Yan-feng,JIN Feng-shuo.Dependence of 5-Fluorouracil inducing cell apoptosis of renal cell carcinoma on Fas/FasL pathway[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(13). |
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| Authors: | WU Guo-ying LI Qian-sheng NIE Zhi-lin LI Yan-feng JIN Feng-shuo |
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| Institution: | WU Guo-ying,LI Qian-sheng,NIE Zhi-lin,LI Yan-feng,JIN Feng-shuo (Department of Urology,Research Institute of Surgery,Daping Hospital,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective To study the roles of Fas/FasL pathway in apoptosis of renal cell carcinoma (RCC) cells induced by 5-Fluorouracil (5-FU). Methods The effects of drug 5-FU in regulating the expressions of Fas and FasL in RCC cell line 786-0 and the effects of 5-FU, anti-Fas monoclonal antibody, CH11, and neutralizing anti-FasL monoclonal antibody NOK-2 on the apoptosis and proliferative activity of RCC cell line 786-0 were studied by Western blot, TUNEL, and MTT techniques. Results Expression of the protein produc... |
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| Keywords: | Fas/FasL |
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