Lack of effect of strain type on detection of toxigenic Clostridium difficile by glutamate dehydrogenase and polymerase chain reaction |
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Authors: | Goldenberg Simon D Gumban Maria Hall Anthony Patel Amita French Gary L |
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Affiliation: | Directorate of Infection, Guy's & St. Thomas' NHS Foundation Trust, London SE1 7EH, UK. simon.goldenberg@kcl.ac.uk |
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Abstract: | Glutamate dehydrogenase (GDH) is popular as a preliminary test for the detection of Clostridium difficile. Recent work has suggested that GDH sensitivity may vary according to ribotype and may be lower for ribotypes 002, 027, and 106 compared with polymerase chain reaction (PCR). We investigated this effect using a dilution series of 64 isolates tested by GDH and Cepheid GeneXpert PCR. PCR was significantly more sensitive than GDH overall; however, there was no difference in detection according to specific ribotype. |
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Keywords: | Clostridium difficile Glutamate dehydrogenase Polymerase chain reaction Laboratory techniques and procedures |
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