The generation of iPS cells using non-viral magnetic nanoparticle based transfection |
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Authors: | Lee Chang Hyun Kim Jung-Hyun Lee Hyun Joo Jeon Kilsoo Lim HyeJin Choi Hye yeon Lee Eung-Ryoung Park Seung Hwa Park Jae-Yong Hong Sunghoi Kim Soonhag Cho Ssang-Goo |
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Affiliation: | Department of Animal Biotechnology and Animal Resources Research Center, Konkuk University, Seoul, 143-701, Republic of Korea. |
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Abstract: | Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transfection method that employs biodegradable cationic polymer PEI-coated super paramagnetic nanoparticles (NP). Our findings support the possible use of transient expression of iPS genes in somatic cells by magnet-based nanofection for efficient generation of iPS cells. Results of dynamic light scattering (DLS) analysis and TEM analyses demonstrated efficient conjugation of NP with iPS genes. After transfection, nanofection-mediated iPS cells showed ES cell-like characteristics, including expression of endogenous pluripotency genes, differentiation of three germ layer lineages, and formation of teratomas. Our results demonstrate that magnet-based nanofection may provide a safe method for use in generation of virus-free and exogenous DNA-free iPS cells, which will be crucial for future clinical applications in the field of regenerative medicine. |
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