| 肝癌标志物高尔基体蛋白73基因在杆菌中的表达 |
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| 引用本文： | 张向颖,刘芳,谢立,娄金丽,刘凯,陈德喜,修冰水,张贺秋.肝癌标志物高尔基体蛋白73基因在杆菌中的表达[J].北京医学,2011,33(12):977-980. |
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| 作者姓名： | 张向颖 刘芳 谢立 娄金丽 刘凯 陈德喜 修冰水 张贺秋 |
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| 作者单位： | 首都医科大学附属北京佑安医院北京市肝病研究所分子生物学室,100069;北京,军事医学科学院基础医学研究所 |
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| 摘 要： | 目的 克隆、表达和鉴定肝癌标志物高尔基体蛋白73 (GP73)基因序列(1028～1327 bp).方法 应用Biosun生物学软件分析GP73全序列,预测其抗原表位,将GP73蛋白基因克隆到表达载体PGEX-4T-2上,构建重组表达质粒PGEX-4T-2/GP73,转化大肠杆菌BL21,异丙基-β-D-硫代半乳糖吡喃...
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| 关 键 词： | 高尔基体蛋白73 克隆表达 |
Expression of the golgi protein 73 for the marker of hepatocellular carcinoma E.coli |
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| Institution: | ZHANG Xiang-ying, LIU Fang, XIE Li, et al (Molecular Biology Laboratory, Beijing Institutes of Liver Disease; Beijing You’an Hospital, Capital Medical University, Beijing 100069) |
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| Abstract: | Objective To clone and express the recombinant golgi protein 73(GP73) and make preparations for antibody preparation and detection. Methods The amino acid sequences of the GP73 were analyzed and aligned using the Biosun software. The gene of antigen epitope was cloned into PGEX-4T-2 vector to construct the recombinant plasmid PGEX-4T-2/ GP73. Recombinant GP73 protein was expressed in E.coli and was purified by GST QZT 4FF. The reaction was evaluated by Western blot and ELISA. Results The recombinant capsid gene could be over experssed in E.coli and had well antigenicity. Conclusion The capsid protein GP73 is successful cloned and expressed, which could be useful for developing antibody preparation and diagnose reagent for HCC. |
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| Keywords: | Golgi protein 73(GP73) Clone and express |
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