原花青素诱导前列腺癌PC-3细胞凋亡的机制研究

目的：探讨原花青素在体外诱导人前列腺癌PC-3细胞凋亡的机制。方法：体外培养人前列腺癌PC-3细胞株，用100，200，300μg／ml原花青素作用24h。荧光素标记的连接素V（Annexin V—fluoresein isothiocyanate，Annexin V—FITC）和碘化吡啶（propidium iodide，PI）双染检测原花青素对PC-3细胞凋亡的影响，采用流式细胞术检测以不同浓度原花青素作用后PC-3细胞线粒体膜电位（△ψm）的变化。结果：100μg／ml原花青素作用细胞24h，5．64％的细胞出现早期凋亡，84．7％的P03细胞呈现线粒体膜电位降低；300vg／ml原花青素组P＆3细胞凋亡率和坏死率分别为44．86％、50．81％。结论：源花青素可在体外诱导PC-3细胞凋亡，其机制与降低线粒体膜电位有关。

A Study on Apoptosis in Prostate Cancer Cells Line PC-3 Induced by Procyanidins

Objective： To observe the effect of procyanidins on apoptosis in cultured prostate carcinoma PC-3 cells and its correlation with mitochondrial membrane potential （△ψm） changes. Methods. After treated with procyanidins 100; 200, 300 μg/ml respectively, PC-3 cells were doubly stained by Annexin V-fluoresein isothiocyanate （FITC） and propidium iodide （PI）. The procyanidins-induced apoptosis and necrosis were detected by flow cytometry（FCM）. The change of △ψm was analyzed with FCM after Rhodamine 123 staining Results： After treated with procyanidins 100μg/ml for 24 hours, the apoptosis rate of PC-3 cells was 5.64%, and △ψm was significantly decreased in 84.7% of PC-3 cells. The apoptosis rate and necrosis rate of PC-3 cells was 44.86 %, 50.81% in the group treated with procyanidins 300μg/ml, respectively. Conclusion. Procyanidins could induce apoptosis and necrosis in PC-3 cells, which might relate with down-regulation of mitochondrial △ψμm.