| 人C1q/肿瘤坏死因子相关蛋白-1高特异性抗体的制备 |
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| 引用本文： | 李洪波,吴东海.人C1q/肿瘤坏死因子相关蛋白-1高特异性抗体的制备[J].国际药学研究杂志,2014,41(2):216-220. |
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| 作者姓名： | 李洪波 吴东海 |
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| 作者单位： | 李洪波 (怀化学院生命科学系民族药用植物资源研究与利用湖南重点实验室,怀化,418000)； 吴东海 (中国科学院广州生物医药与健康研究院,广州,510530)； |
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| 基金项目： | 国家自然科学基金资助项目(项目编号：81300655)湖南省教育厅优秀青年基金资助项目(项目编号：12B100)湖南省高校科技创新团队支持计划(项目编号：2010212)湖南省重点学科建设资助项目(项目编号：2011042) |
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| 摘 要： | 目的 制备人补体C1q/肿瘤坏死因子相关蛋白1（hCTRP1）的特异性抗体.方法 合成了hCTRP1的C端抗原肽并免疫新西兰大白兔,利用亲合纯化色谱柱对抗体进行了纯化.结果 间接ELISA测定表明,抗血清效价达1∶64 000,Western印迹及ELISA验证表明纯化抗体具有很高的特异性.结论 人工合成的hCTRP1抗原肽免疫新西兰大白兔,制备了高效价的抗血清,并纯化制备了hCTRP1特性抗体.
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| 关 键 词： | 人C1q 肿瘤坏死因子相关蛋白1 抗原肽 多克隆抗体 亲合纯化 |
Preparation of high specific antibody against human C1q/TNF-related protein-1 |
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| LI Hong-bo,WU Dong-hai.Preparation of high specific antibody against human C1q/TNF-related protein-1[J].Foreign Medical Sciences(Section of Pharmarcy),2014,41(2):216-220. |
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| Authors: | LI Hong-bo WU Dong-hai |
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| Institution: | 1. Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province Department of Life Sciences, Huaihua College, Huaihua 418008, China; 2. Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China) |
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| Abstract: | Objective To prepare specific antibody against human Clq/TNF-related protein-1 （hCTRP1）. Methods A C- terminal antigenic peptide of hCTRP1 was synthesized and injected into the Newzealand rabbits. The antibody was purified by affinity chromatography column. Results High titer antisera against the hCTRP1 was prepared and the antiserum＇s titration was over 1 ： 64 000 by indirect ELISA. The specificity of the purified antibody by affinity chromatography was identified by Western blot and ELISA. Conclusion The high titer antiserum is prepared by injection for rabbits and the specificity of the antibody purified from the antiserum is determined. |
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| Keywords: | human C1q/TNF-related protein-I antigenic peptide polyclonal antibody affinity purification |
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