新型含锶硫酸钙的制备及其生物相容性试验

目的制备一种新型含锶硫酸钙材料并对其进行生物相容性评价。方法探讨采用水热法制备一种新型的含锶硫酸钙并对制备的材料进行：（1）X线衍射、傅里叶变换红外光谱及差式量热扫描表征检测，以验证材料为目标产物；（2）细胞毒性试验评估材料浸提液对L-929小鼠成纤维细胞形态学及相对增殖率的影响；(3)溶血试验评估材料浸提液的血液相容性；（4）体内植入试验评估其对活体组织局部毒性反应。结果（1）X线衍射结果示:14.63°，25.72°和29.80°处可见α-半水硫酸钙的特征峰，在24.78°处可见锶元素的特征峰;傅里叶变换红外光谱与α-半水硫酸钙标准图谱相似；差式量热扫描结果示：制备材料中结构水含量为6.03%；（2）细胞毒性试验显示被试材料浸提液在不同时间点的毒性反应均为I级，无细胞毒性；（3）溶血试验结果显示溶血率为4.3%，符合溶血试验要求；（4）体内植入结果表明材料和机体达到稳定状态。结论该材料为含锶硫酸钙，生物相容性试验表明其具有良好的生物相容性。

Preparation and biocompatibility of a novel strontium-containing calcium sulfate

Objective To prepare a novel strontium-containing calcium sulfate and assess its and biocompatibility. Methods A novel strontium-containing α-calcium sulfate hemihydrate (Sr-caS) bone substitute as prepared with hydrothermal reaction and examined for X-ray diffraction (XRD), Fourier transform infrared (FTIR) and thermogravimetric differential scanning calorimetric (TG-DSC) patterns. The biocompatibility of the material was evaluated by in vitro cytotoxicity test in L-929 cells, hemolysis test of blood, and in vivo implantation test in SD rats. Results The XRD spectra of the prepared Sr-CaS powder highlighted 3 strong characteristic peaks ofα-CaSO4 at 14.63° , 25.72° and 29.80° with a strontium-specific peak at 24.78° . The FTIR patterns of Sr-CaS resembled those of CaS. TG-DSC results showed that the material contained a non-evaporable water content of 6.03%. In vitro cytotoxicity test in L-929 cells suggested that the material had a class 1 cytotoxicity, and the hemolysis rate of its aqueous extract was 4.3%. The material implanted in the muscular tissues of SD rats maintained a steady state in the surrounding tissues. Conclusion This strontium-containing calcium sulfate material we prepared shows an excellent biocompatibility for potential use as a novel artificial bone material.