虎杖苷对ApoE-/-小鼠肝脏miR-214表达水平及肝功能的影响 |
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引用本文: | 周凤华,温子云,何泽淮,李梅,尹琼丽,石成钢,成彩联.虎杖苷对ApoE-/-小鼠肝脏miR-214表达水平及肝功能的影响[J].南方医科大学学报,2016,36(6):763-767. |
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作者姓名: | 周凤华 温子云 何泽淮 李梅 尹琼丽 石成钢 成彩联 |
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作者单位: | 1. 南方医科大学中医药学院,广东 广州,510515;2. 中山大学附属第三医院 特诊医疗中心,广东 广州,510630;3. 中山大学附属第三医院 肾脏内科,广东 广州,510630 |
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基金项目: | 国家自然科学基金青年基金(81403339);广东省自然科学基金博士启动项目(2014A030310150);广东省中医药管理局科研项目(20141186) Supported by Natural Science Foundation for the Youth (NSFY) of China (81403339) |
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摘 要: | 目的研究虎杖苷(PD)对ApoE-/-小鼠肝脏miR-214及肝功能的影响。方法采用高脂饲料喂养雄性ApoE-/-小鼠,建立小鼠
AS模型。将ApoE-/-小鼠随机分为4组(n=10),即模型组(Model)、辛伐他汀组(Simvastatin)、PD低剂量组(PDL)、PD高剂量组
(PDH),另设10只同龄C57BL/6J小鼠为正常对照组。连续给药12周后取血,检测小鼠血糖、血脂、谷草转氨酶(AST)、谷丙转
氨酶(ALT)、肝脏T-SOD及MDA等指标,HE染色观察肝组织病理切片,实时荧光定量PCR检测miR-214水平。结果模型组小
鼠血糖和血清中TC、TG、LDL-C、ALT和AST,以及肝脏中MDA较正常对照组显著增高(P<0.01),血清中HDL-C和肝脏中
T-SOD、miR-214则显著降低(P<0.01),肝切片可见细胞内充满大小不一脂滴,大部分肝细胞呈现脂肪变性;与模型组相比,PD
高剂量组能显著降低小鼠空腹血糖、血清TC、TG、LDL-C、AST、ALT以及肝脏中的MDA(P<0.05),并且能够显著升高血清
HDL-C和肝脏miR-214及T-SOD(P<0.05);PD高、低剂量组小鼠肝细胞脂肪变性与模型组相比均有所减轻。结论PD能降低
AS小鼠血糖、血脂,并保护肝功能,其机制可能主要与PD升高肝脏miR-214水平,调节T-SOD与MDA等氧化应激指标有关。
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关 键 词: | 虎杖苷 动脉粥样硬化 miR-214 肝功能 氧化应激 |
Effect of polydatin on miR-214 expression and liver function in ApoE-/-mice |
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Abstract: | Objective To study the effect of polydatin on the expression level of miR-214 and liver function in atherosclerotic mice. Methods Forty male ApoE-/- mice were randomly allocated into 4 groups (n=10), namely the model group, low- and high-dose polydatin groups, and simvastin group, with 10 male C57BL/6J mice serving as the normal control group. Mouse models of atherosclerosis were established by feeding the ApoE-/-mice with a high-fat diet. After 12 weeks of treatment, blood levels of glucose, lipids, AST, and ALT and the contents of T-SOD and MDA in the liver tissue were detected. The pathologies of the liver were examined with HE staining, and miR-214 expression in the liver was detected using quantitative real-time PCR. Results Compared with the normal control mice, the mice in the model group showed significantly increased blood glucose, serum TC, TG, LDL-C, ALT, and AST levels, and MDA contents in the liver (P<0.01), with significantly decreased serum HDL-C level and SOD and miR-214 levels in liver (P<0.01). Polydatin treatment significantly ameliorated such changes in blood glucose, serum ALT, AST, TC, TG, LDL-C, and HDL-C levels, and MDA, SOD, and miR-214 contents in liver tissue (P<0.05). Conclusions Polydatin can reduce blood glucose and lipid levels and protect the liver function in atherosclerotic mice possibly by up-regulating the expression of miR-214 and T-SOD and down-regulating MDA in the liver. |
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Keywords: | polydatin atherosclerosis miR-214 liver function oxidative stress |
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