Ras和Sos1蛋白在上皮性卵巢恶性肿瘤组织中的表达及临床意义

目的检测Ras 和Sos1 蛋白在上皮性卵巢恶性肿瘤中的表达，分析两种蛋白表达水平与上皮性卵巢恶性肿瘤临床病理指标的相关性。方法利用免疫组织化学SABC法检测62 例上皮性卵巢恶性肿瘤组织、5 例交界性上皮性卵巢肿瘤、15 例卵巢上皮性囊腺瘤、18 例正常卵巢组织中Ras 与Sos1 蛋白的表达情况，统计学分析两种蛋白表达水平与肿瘤恶性程度指标的相关性。结果（1）Ras 和Sos1 在上皮性卵巢恶性肿瘤组织中的表达水平显著（P<0.05）高于在其它组织中的表达；（2）两种蛋白在上皮性卵巢恶性肿瘤细胞中的表达部位均以细胞膜和细胞浆同时表达为主；（3）Ras 的表达程度（光密度值）与病理分型有相关性（P<0.05）：其在浆液性卵巢癌的表达比在其他病理类型的表达更为显著，而Sos1 蛋白表达与这些病理参数无相关性；（4）两种蛋白表达程度高的患者无瘤生存时间短，但不具有显著性。结论Ras、Sos1 这两种蛋白与上皮性卵巢恶性肿瘤的发生和发展可能存在内在联系；Ras 蛋白的表达存在组织类型的差异，可用来支持卵巢浆液性腺癌的特异性诊断；统计学分析显示，两种蛋白表达程度高的患者，无瘤生存时间较低表达患者更短，但可能由于病例数较少有关，该结果不具备显著性，这需要扩大样本量进一步研究。

Expressions of Ras and Sos1 in epithelial ovarian cancer tissues and their clinical significance

Objective To detect the expressions of Ras and Sos1 proteins in human epithelial ovarian cancer (EOC) tissues andexplore their correlation with the clinicopathological features of the patients. Methods The expressions of Ras and Sos1proteins were detected immunohistochemically in 62 EOC tissues, 5 borderline ovarian cancer tissues, 15 benign epithelialovarian neoplasm tissues, and 18 normal ovarian tissues. Results The EOC tissues showed significantly higher expressionlevels of both Ras and Sos1 than the other tissues tested (P<0.05). In EOC tissues, Ras and Sos1 proteins were expressed mostlyon the cell membrane and in the cytoplasm. The expression level of Ras was correlated with pathological types of the tumor (P<0.05) and was the highest in serous cystadenomcarcinoma; Sos1 expression did not show significant correlation with theclinicopathological indexes of the patients. High expressions of both Ras and Sos1 proteins were associated with shorterprogression-free survival of the patients, but this association was not statistically significant. Conclusions Ras and Sos1 proteinmay participate in in the occurrence and development of EOC. The tissue-specific variation of Ras expression can lend supportto a specific diagnosis of ovarian serous adenocarcinoma. The association of Ras and Sos1 protein expression with thetumor-free survival time of the patients awaits further investigation with a larger sample size.