SD大鼠钳夹脊髓损伤模型的建立

目的探讨构建一种SD大鼠脊髓钳夹损伤模型，用于脊髓损伤（spinal cord injury，SCI）的研究。方法将36只成年雄性SD大鼠随机分成A、B、C三组，每组12只。脊髓损伤组（A组）：用动脉瘤夹钳夹大鼠第10胸椎对应脊髓。假手术组（B组）：只打开椎板，暴露脊髓，不造成SCI。正常对照组（C组）：正常大鼠，不做任何处理。通过行为学观察（BBB评分）、HE染色和神经电生理检测进行判断。结果HE染色结果：A组可见脊髓正常组织被破坏，损伤区可见血管破裂出血，有大片坏死灶，周围神经细胞肿胀变性，内有炎症细胞浸润。B、C两组基本一致，组织结构正常。BBB评分：B组术后1周功能恢复接近正常，评分20分。A组术后第2周开始恢复，到第3周基本停止，最终BBB评分低于6分，两组比较有明显差异（P〈0．05）。神经电生理（SEP）检测：A组可以明显看到SEP的波峰趋于水平，且潜伏期无限延长，与B组相差显著（P〈0．05）。A组内动物BBB评分、SEP无显著性差异（P〉0．05）。结论通过动脉瘤夹钳夹SD大鼠T10脊髓，能够建立良好的脊髓损伤动物模型。

Establishment of spinal cord injury model with ring clamp in SD rats

Objective To establish spinal cord injury （SCI） model of SD rats at Tlo with ring clamp and to provide reliable data for fur- ther study. Methods 36 male SD rats were randomly divided into 3 groups （ 12 rats per group）. SCI group（ group A） ：the spinal cord of the rats were clamped on at Tl0 by aneurysm clip. Sham group （group B ） ：merely opened the neural scute and the spinal cord was exposed without injury. Normal controlled group（ group C ） ： normal rats without any arrangement. Ethological study （BBB scores）, histological and electrophys- iological tests were conducted. Results HE staining results in group B and group C were identical as normal. Fragmented structure and nec- rosis could be seen in white and grey matter,and cellular swelling was detected in injured region in group A. BBB scores between group A and group B was significantly different （ P 〈 0.05 ）. Electrophysiological（SEP） demonstrated that peak-to-peak value trended horizon and la- tency was extended infinitly in group A , the difference between group A and group B was significant （ P 〈 0.05 ）. Conclusion A spinal cord injury model of SD rats can be established by clamped on T10 with aneurysm clip.