荧光定量PCR检测病理组织中结核分枝杆菌的临床价值

目的探讨应用荧光定量PCR（FQ-PCR）技术在病理组织中检测结核分枝杆菌DNA（TB-DNA）的临床应用价值。方法对196例临床诊断结核的穿刺病理活检标本进行石蜡包埋切片，采用实时荧光定量PCR技术检测TB．DNA，并行抗酸染色及组织病理学检查，对三种检测与临床诊断符合率进行比较。结果在196例临床诊断结核的石蜡包埋组织中，荧光定量PCR检测阳性136例，阳性率为69．39％，抗酸染色检测阳性69例，阳性率为35．20％，组织病理学检查阳性102例，阳性率为52．04％，荧光定量PCR检测阳性率最高。结论荧光定量PCR技术简便、快捷，敏感性强、特异性高，可作为结核病分子病理诊断的重要检测方法，具有较高的临床应用价值。

Clinical value of quantitative PCR technology for detection of mycobacterium tuberculosis in pathological tissue

Objective To explore the application of fluorescent quantitative PCR（FQ-PCR） and its clinical value for histopathological detection of mycobacterium tuberculosis DNA（TB-DNA）. Methods The paraffin-embedded needle biopsy sections from 196 cases with tu- berculosis were detected by quantitative PCR technique with TB-DNA in real time, and acid-fast staining and histopathological examinations were conducted at the same time. Results Among 196 cases with the clinical diagnosis of tuberculosis in paraffin-embedded tissues, there were 136 cases（69.39% ） screened positive by fluorescence quantitative PCR, 69 cases （35.20%） screened positive by acid-fast staining examination, and 102 cases（52.04% ） screened positive by histopathological examination. The fluorescence quantitative PCR detection is of the highest positive rate. Conclusion The fluorescence quantitative polymerase chain reaction technology is simple, efficient, and of sensitiv- ity and high specificity. Since it is of high clinical value, it can be used as a pathological diagnostic method for tuberculosis molecular detec- tlon