非胰岛素依赖型糖尿病病人红细胞形态变化及其影响因素的观察

①目的观察非胰岛素依赖型糖尿病(NIDDM)病人红细胞形态变化及其影响因素。②方法观察64例NIDDM病人外周血红细胞扫描电镜形态,同时检测了红细胞膜胆固醇(Ch)、磷脂(PL)、脂质过氧化物(LPO)含量的变化,并与39例健康人进行对照。⑧结果NIDDM病人异常红细胞百分率明显高于对照组(t＝4.826,p<0.001),出现口形、棘形、嵴形、球形等红细胞。NIDDM病人红细胞膜Ch,Ch/PL和LPO含量明显增高,而PL明显降低,与对照组比较,差异有极显著性(t＝3.765~4.829,p均<0.001)。有血管病变者各指标变化较无血管病变者更明显(t=4.126~5.987,p均<0.001)。NIDDM病人红细胞异常率与Ch,Ch/PL和LPO呈正相关(r＝0.461~0.506,p均<0.001),与PL呈负相关(r＝-0.378,P<0.001)。④结论NIDDM病人红细胞膜脂质成分异常是影响红细胞形态的重要因素之一。

STUDY OF ERYTHROCYTE MORPHOLOGY AND AFFECTING FACTORS IN PATIENTS WITH NON-INSULIN DEPENDENT DIABETES MELLITUS

Objective To study the morphological changes of erythrocyte and its affecting factors in patients with non-in-sulin dependent diabetes mellitus(NIDDM). Method The erythrocyte morphology, the membrane cholesterol(Ch), phospho-lipid(PL) and liperoxide(LPO) were studied in 64 NIDDM patients, and compared with 39 healthy persons. Result The abnormal morphology rate was significantly higher in the patients than in the controls(t = 4. 826, P<0. 001), and there were stomato-cyte, acanthocyte, ridgecyte, spherocyte erythrocyte in peripheral blood. The content of erythrocyte membrane Ch, Ch/PL and LPO were significantly higher, PL was significantly lower in the patients than in the controls(t = 3. 765~4. 829, P<0. 001). The changes of the above indicators in NIDDM patients with angiopathy were more obvious than in patients without angiopathy (t = 4. 126~5. 987, P<0. 001) The morphology abnormal rate was positively correlated to Ch, Ch/PL and LPO(r=0. 461 - 0. 506, P< 0. 001), negatively correlated to PL(r= - 0. 378, P<0. 001). Conclusion The lipid abnormalities of erythrocyte membrane in NIDDM patients are important factors affecting the erythrocyte morphology.