不同CO2压强对MKN-28细胞黏附侵袭能力的影响

目的：探讨不同CO2压强及时程对MKN-28细胞黏附侵袭能力的影响。方法：利用密封箱建立体外气腹模型，选用胃癌细胞株MKN-28，分别在9 mmHg(1 mmHg=0.133 kPa)和15 mmHg压强下以及常规条件下暴露2 h和4 h后，用CytoMatrixTM细胞黏附试剂盒检测细胞黏附能力，流式细胞技术(FACScan)检测表达E-钙黏蛋白(E-cadherin)和细胞间黏附分子(Intercellular adhesion molecule-1，ICAM-1)的细胞百分数。另将处理后的MKN-28细胞注入裸鼠腹腔(2×106个细胞/只)，每组10只。4周后每组取5只处死，记录腹腔成瘤情况，剩余裸鼠观察生存时间。结果：MKN-28细胞株经不同压强与不同时程的CO2处理后，胃癌细胞MKIN-28黏附能力，表达E-cadherin和ICAM-1的细胞百分数MKN-28的腹腔内转移个数，生存天数，裸鼠腹腔种植成瘤数和生存时间各组间均差异均无统计学意义(P＞0.05)。结论：在不高于15 mmHg压强，且不超过4 h情况下，不同CO2压强及不同时程对MKN-28细胞株的黏附侵袭能力并无显著影响，亦不增加肿瘤的转移几率。

Effects of different CO2 pressures on adhesive and invasive ability of MKN-28 gastric carcinoma cells

ObjectiveTo study the effects of different CO2 pressures on adhesive and invasive alility of MKN-28 gastric carcinoma cells. MethodsWith an artificial pneumoperitoneum model in vitro, MKN-28 human gastric carcinoma cells were exposure to CO2 in 3 different pressure groups: 9mmHg, 15mmHg and control group. The adhesive ability of MKN-28 cells before and after exposed to CO2 was detected by CytoMatrixTM kit. The percentage of cells expressing E-cadherin and ICAM-1 among MKN-28 cells were counted by flowcytometry in vitro. All the MKN-28 gastric carcinoma cells were injected into abdominal cavity of nude mice(2×106cells/mouse) respectively. Five mice of each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity.The remaining mice were kept to observe the survival time. ResultsIn the 3 groups,all of the adhesive ability of MKN-28 cells (cd/m2). the percentage of cells expressing E-cadherin,and ICAM-1; the number of intra-abdominal tumor metastases. the survival time and the tumor metastasis in abdominal cavity of nude mice were no sigmificant difference call (P＞0.05). ConclusionsWhen the pressure is below is below 15 mmHg and for less thqn 4 h, different CO2 pressures and different time intervals do not affect the adhesive and invasive ability of MKN-28 cells in the abdominal cavity,and not increase the chancws of tumonr metastasis.