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| 苯乙酸对胶质瘤细胞增殖周期的抑制 | |
| 引用本文： | 田宇,赵兴利,陈宝琴,田洋,赵丛海. 苯乙酸对胶质瘤细胞增殖周期的抑制[J]. 中国耳鼻咽喉颅底外科杂志, 2000, 6(2): 65-67 |
| 作者姓名： | 田宇 赵兴利 陈宝琴 田洋 赵丛海 |
| 作者单位： | 1. 白求恩医科大学三院中日联谊医院 神经外科,吉林 长春 130031 2. 吉林省人民医院,吉林 长春 130021 3. 吉林省中医中药研究院 外科,吉林 长春 130021 |
| 基金项目： | 国家自然科学基金资助项目(39900152);吉林省科委基金资助项目(970567-4) |
| 摘 要： | 目的观察诱导分化剂苯乙酸对胶质瘤细胞C6增殖抑制作用及对细胞周期的影响.方法细胞计数及[3H]-TdR掺人法检测苯乙酸对C6细胞的增殖抑制作用.FCM检测苯乙酸对C6细胞周期的影响.结果C6细胞增殖与苯乙酸剂量及应用时间呈负相关,苯乙酸2.5,5.0mmol/L组用药第5d,对C6细胞的增殖抑制率为74.7%,95.0%;第ld,3d上述两组用药组G0/G1期比例分别为45.1%,56.2%和31.3%,31.4%.对照组为56.3%.58.2%.结论苯乙酸对C6细胞存在剂量和时间依赖性抑制,使细胞周期抑制于G1期. |
| 关 键 词： | 苯乙酸 神经胶质瘤 病理生理 细胞增殖 细胞周期 |
| 文章编号： | 1007-1520(2000)02-0065-03 |
| 修稿时间： | 1999-09-23 |
Inhibition to the cell cycle of glioma cell C6 by phenylacetate |
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| TIAN Yu,ZHAO Xing li,CHEN Bao qin,et al.. Inhibition to the cell cycle of glioma cell C6 by phenylacetate[J]. Chinese Journal of Otorhinolaryngology-skull Base Surgery, 2000, 6(2): 65-67 | |
| Authors: | TIAN Yu ZHAO Xing li CHEN Bao qin et al. |
| Abstract: | Objective To determine the growth-inhibitory activity of phenylacetate (PA) on glioma cell line C6 and to find the influence of the cell cycle by PA. Methods The effect of PA on cell proliferation was determined with [ 3H]-TdR assay, and cell cycle distribution analysis was done with flow cytometry. Results At the dose ranges of 2.5 and 5.0 mmol/L, PA caused a dose-dependent and time-dependent proliferation inhibitory effect. Cells arrested in Phases G0/G1 were observed in PA-treated ( 2.5 , 5.0 mmol/L) cells. Conclusion PA causes a dose-dependent and time-dependent proliferation inhibitory effect, and the main effect of PA is the arrest of glioma cells in Phase G1. |
| Keywords: | PHENYLACETATE/pharm GLIOMA/physiopathol CELL CYCLE/drug eff |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |