伊那普利拉对新生鼠心肌成纤维细胞增殖及作用机制的研究

目的 观察血管紧张素I转换酶抑制剂（angiotensin I converting enzyme inhibitor,ACEI）伊那普利拉（enal-aprilat,Ena）抗血管紧张素Ⅱ（angiotensinⅡ,AngⅡ）诱导的心肌成纤维细胞（CFb）增殖及其对细胞周期蛋白的影响,探讨Ena抗CFb增殖及机制。方法以培养的新生wistar大鼠乳鼠CFb为实验模型,实验分为对照组,AngII组,用药组3个剂量,采用胰酶消化、差速贴壁法培养CFb,四氮唑盐（MTT）比色法检测CFb增殖;羟脯氨酸法测定胶原量;流式细胞仪测定细胞周期和细胞周期蛋白依赖性激酶抑制剂p^27kip1（Cyclin dependent kinase inhibitor,CKI）表达。结果Ena能够抑制AngⅡ诱导的CFb增殖（P〈0.01,P〈0.05）,降低羟脯氨酸含量（P〈0.01,P〈0.05）,增加细胞周期中G0/G1期百分率,降低S期百分率,提高p27kip1表达（P〈0.01,P〈0.05）。结论Ena抑制AngII诱导的CFb增殖与提高p^27kip1表达有关。

Research of enalaprilat on neonatal rat cardiac fibroblasts proliferation and its mechanism

Objective To investigate the effects of angiotensin - converting enzyme inhibitor Ena on proliferation and cell cycle protein of cardiac fibroblasts and to explore its mechanism on inhibiting cardiac fibrosis. Methods CFb was isolated by trypsin digestion method. MTT colorimetric assay was adopted to evaluate cell proliferation,collagen synthesis were observed by hydroproline concentration method, flow cytometry was used to measure cell cycle, immunocytochemical staining method to test p^27kip1expression. Results Ena decreased MTT value and hydroproline content dramatically, increased phase G0/G1 and decreased phase S percentage ratio of cell cycle, elevated p^27kip1 expression. Conclusion The effects of Enalaprilat on inbibiting CFb proliferation can be attributed to increasing p^27kipl expression.