Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues. |
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Authors: | A Krishan A Oppenheimer W You R Dubbin D Sharma B L Lokeshwar |
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Affiliation: | Division of Experimental Therapeutics, Sylvester Cancer Center, University of Miami School of Medicine, Florida 33136, USA. |
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Abstract: | Androgen receptor (AR) plays an important role in growth and hormonal therapy of human prostate tumors. Immunohistochemical analysis of AR expression, a nonquantitative technique, is currently used for screening of receptor expression in prostate tissues. The present report describes a laser flow cytometric method for monitoring AR expression in human cell lines and in archival formalin-fixed paraffin-embedded prostate tissues and tumors. Multiparametric flow analysis can be used for simultaneous detection of other cellular markers (e.g., DNA aneuploidy), and by gated analysis, AR expression in subpopulations of a tumor can be quantitatively determined. |
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