Use of B-IV zonal rotor centrifugation as a simple tool for the separation of adeno-associated X7 virus (AAV X7) from helper adenoviruses |
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| Authors: | E. Luchsinger R. Strobbe D. Dekegel G. Wellemans |
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| Affiliation: | (1) Instituto de Microbiologia, Universidad Austral de Chile. Valdivia Scolarship, Ministère de l'Education National et de la Culture, Brussels, Belgium;(2) Laboratory for electron microscopy, Pasteur Institute, Brussels, Belgium;(3) Present address: Institut National de Recherches Vétérinaires, Belgium |
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| Abstract: | Summary The use of zonal rotor centrifugation in the separation of AAV X7 virus from its helper adenovirus is reported. AAV X7 virus was perfectly separated from its helper adenovirus. Adeno-associated X7 virus was found to separate in par tides with an  S rate of 72–82 S, and a density in CsCl of 1.30–1.32, and virions with a sedimentation coefficient of 135–147 Svedbergs, with a density of 1.37–1.38. |
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