石蜡切片研究H9N2亚型禽流感病毒的免疫原性

目的 用石蜡切片评估龠流感病毒A／Chicken／Guangdong／SS／94(H9N2)株的免疫原性，以获得免疫原性良好的疫苗候选株。方法 以A／CKcken／Guangdong／SS／94(H9N2)株为抗原制备油乳剂灭活疫苗免疫1Od龄SPF鸡，免疫21d后，以该毒株静脉注射免疫组与非免疫对照组，定期剖杀试验鸡并取脑、心肌、肺、胰腺、肝、肾、脾、气管和法氏囊等组织用石蜡切片进行病理组织学检查。结果 两组试验鸡的的病理组织学变化有明显差异，免疫组试验鸡获得保护，而对照组病变明显。结论 A／CKcken／Guangdong／SS／94(H9N2)株的免疫原性良好，可作为制备油乳剂灭活疫苗的候选株。

Studies on the immunogenicity of avian influenza virus strain of A/Chicken/Guangdong/SS/94(H9N2) by paraffin section

To get a good vaccine candidate,the immunogenicity of Avian Influenza Virus (AIV) strain of A/Chicken/Guangdong/SS/94(H9N2) was evaluated by paraffin section.The strain of A/Chicken/Guangdong/SS/94(H9N2) was used as antigen and established into an inactivated oil-emulsion vaccine and vaccinated 10-day-old SPF chickens.Both vaccinated and unvaccinated chickens were challenged with this strain via infravenous injection after 21 days of vaccination.Chickens of the two groups were killed at scheduled time and the brain,cardiac muscle,lung,pancreas,liver,kidney,spleen,trachea and bursa were collected and established paraffin section for investigation of their pathologic histology.Pathologic histology changes of the two groups were distinctly,the vaccinated chickens were protected while the unvaccinated chickens had apparently pathological changes.It concludes that A/Chicken/Guangdong/SS/94(H9N2) has good immunogenicity and could be used as a vaccine candidate to established an inactivated oil-emulsion vaccine.