复合真菌多糖诱导肝癌Bel-7402细胞凋亡的研究

采用体外细胞培养的方法 ,观察复合真菌多糖诱导人肝癌细胞 Bel- 740 2凋亡的影响 ,利用双重免疫标记方法分析肿瘤抑制基因 P5 3在 Bel- 740 2凋亡中的表达。结果表明 :不同浓度的复合真菌多糖均能明显诱导 Bel- 740 2细胞凋亡 ,并随浓度增加而渐次出现不同时相的细胞凋亡特征性染色质凝聚 ;能明显降低G1 、S期 ,且有显著的剂量 -效应关系 ,凋亡细胞百分率亦随浓度的增加而逐渐升高 ;在高浓度组出现典型的DNA阶梯状电泳条带。证明复合真菌多糖抗肿瘤作用机理与诱导肿瘤细胞的凋亡密切相关 ,其诱导肿瘤细胞凋亡是 P5 3非依赖性。

Study of fungus polysaccharides compounds (FPC) in inducingthe apoptosis of liver cancer cell Bel-7402

To observe the influence of fungus polysaccharides compounds (FPC) in inducing human liver cancer cell Bel 7402 apoptosis in cell cultivating in vitro, the authors analyzed tumor inhibitive gene P53 expression in Bel 7402 apoptosis by applying double immuno marker. The result showed that the multilevel of FPC could all apparently induce Bel 7402 apoptosis. With the enhancement of FPC concentration, the authors observed chromatin condensation in some phases companying with the characteristic apoptosis. In the meantime, it could also greatly reduce the G 1 and S, with obviously dose response relationship. The percentage of cell apoptosis increased with the enhancing of concentration. In the high level group the authors found typical DNA ladder eletrophoresis stripe. The result showed that the mechanism of the FPC antineoplastic effect had an intimate relation with its induction to apoptosis and that the result of FPC inducing tumor cell apoptosis had the character of P53 independence.