| Effects of rHu-EPO on Myocyte Apoptosis and Cardiac Function Following Acute Myocardial Infarction in Rats |
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| 作者姓名： | 叶亮 杜心灵 夏家红 江平 |
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| 作者单位： | [1]DepartmentofCardiovascularSurgery,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,China [2]DepartmentofAnesthesiology,XianningHospitalofTraditionalChineseMedicine,Xianning437100,China |
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| 基金项目： | This project was supported by a grant from Natural Sci ences Foundation of Hubei Province (No. 2002AB105). |
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| 摘 要： | The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infarction in rats were studied. Thirty-two rats were divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery, rHu EPO was administered i.p. in MI EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2, bax) and TUNEL dyeing. The results showed that hemodynamic function in MI.4- EPO group was much better than in MI group.The number of the cells positive for bax and TUNEL in MI EPO group was less than that in MI group. The number of the cells positive for bcl-2 in MI EPO group was more than that in MIgroup. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function.
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| 关 键 词： | rHu-EPO 急性心肌梗死 小鼠 动物模型 心功能 肌细胞 细胞凋亡 |
| 收稿时间： | 11 February 2004 |
Effects of rHu-EPO on myocyte apoptosis and cardiac function following acute myocardial infarction in rats |
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| Ye Liang,Du Xinling,Xia Jiahong,Jiang Ping.Effects of rHu-EPO on Myocyte Apoptosis and Cardiac Function Following Acute Myocardial Infarction in Rats[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(1):55-58. |
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| Authors: | Ye Liang Du Xinling Xia Jiahong Jiang Ping |
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| Institution: | 1. Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2. Department of Anesthesiology, Xianning Hospital of Traditional Chinese Medicine, Xianning 437100, China |
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| Abstract: | The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infarction in rats were studied. Thirty-two rats were divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI+EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery. rHu-EPO was administered i.p. in MI+EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI+EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2, bax) and TUNEL dyeing. The results showed that hemodynamic function in MI+EPO group was much better than in MI group. The number of the cells positive for bax and TUNEL in MI+EPO group was less than that in MI group. The number of the cells positive for bcl-2 in MI+EPO group was more than that in MI group. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function. |
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| Keywords: | recombinant human erythropoietin myocardial infarction apoptosis |
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