尖锐湿疣皮损中HPV类型及p53、bcl—2分子表达研究

目的 探讨尖锐湿疣(CA)中HPV（人乳头瘤病毒）感染类型及其p53和bcl－2（B细胞淋巴瘤样因子2）分子在HPV6／11阳性尖锐湿疣发病中的可能作用。方法 用PCR法检测了22例CA皮损中HPV6／11和HPV16／18，用免疫组化方法检测了尖锐湿疣组织中p53和bcl－2分子的表达。结果 （1）所有标本均为HPV6／11阳性，未检测出HPV16／18；（2）40．9％的尖锐疣皮损在表皮角质形成细胞中有p53蛋白的表达，但阳性细胞数量较少，主要散在分布于基底层；（3）bcl－2蛋白在CA及鳞状细胞癌中未见表达。结论 （1）HPV6／11是CA的主要致病病毒；（2）CA中部分出现p53分子表达提示这部分细胞可能已出现恶性转化倾向；（3）bcl-2可能与角质形成细胞来源的良性增生性疾病以及恶性肿瘤关系不大。

Typing of Human Papillomavirus and Detection of p53 and bcl-2 Molecules Expression in Condyloma Acuminatum

Objective To study the causative HPV types in condyloma acuminatum(CA) and better understand the possible role of p53 and bcl-2 molecules in the pathogenesis of CA.Methods HPV 6/11 DNA and 18/18 DNA were detected in fresh lesions of CA by polymerase chain reaction;The expression of p53 and bcl-2 molecules were determined by immunohistochemistry.Results (1)HPV6/11 was detected in all specimen and no HPV16/18 was found;(2)The expression of p53 was seen in 40.9% of specimen,characterized by scattered positive cells in the basal cell layer;(3)bcl-2 molecule was not found in CA lesion.Conclusion (1)HPV6/11 is the major causative virus in CA;(2)The expression of p53 molecule in some kerotinocytes of CA suggests that CA has a tendency of malignant transformation in the lesions.(3)The bcl-2 molecule may not be involved in keratincoyte-derived hyperplastic diseases and malignances.