外源NGF促进大鼠皮肤创面模型损伤修复的机制研究

目的：探究外源NGF对大鼠创面的治疗作用及机制。方法：在SD雄性大鼠的背部脊柱的两侧手术切开形成两个2 cm × 2 cm的伤口，右边伤口处给予20 mg/ml NGF 1 ml，左边伤口给等量生理盐水作为对照组。隔天给药到第14天（第14天停止给药），分别于d3、d7、d14统计创面伤口愈合率，并取这3个时间点的组织用免疫组化的方法检测转化生长因子（ transforming growth factor-β1，TGF-β1）、CD68-阳性巨噬细胞、增殖性细胞核抗原（proliferating cell nuclear antigen，PCNA）、血管内皮生长因子（vascular endothelial growth factor，VEGF-1）的表达量。结果：NGF组的创面愈合率明显高于生理盐水组，并且NGF组TGF-β1、CD68、PCNA、VEGF-1的蛋白表达量也显著高于生理盐水组（P〈0.05）。结论：外源NGF具有促进创面愈合作用，其机制可能与趋化炎症因子、加快血管生成、促进PCNA、TGF-β1的表达相关。

Study on the mechanism of exogenous nerve growth factor in accelerating the wound healing in the model of rats with skin damage

Objective： To study the therapeutic effect of nerve growth factor （NGF） on wound healing in rats and its mechanism. Methods：Two full thickness wounds （2 cm ×2 cm） extending through the panniculus carnosus were made on both sides of the back spine in SD male rats. The right incision （designated as an experimental group） was treated with 1 ml of NGF （20 mg/ml, dissolved in 0.9%sterile saline） every other day till 14th days while the left incision （designated as a control group） with equal amounts of 0.9%sterile saline. The rates of wound healing were recorded at day 3, 7 and 14 and the expression of transforming growth factor-β1 （TGF-β1）, CD68＋macrophages, proliferating cell nuclear antigen （PCNA） and vascular endothelial growth factor （VEGF-1） in wound tissues sampled at day 3,7 and 14 were detected by immunohistochemistry staining. Results：The rates of wound healing and the protein expression of TGF-β1、CD68、PCNA、VEGF-1 were obviously higher in the experimental group than in the control group （P〈0.05）. Conclusion：The mechanism of NGF in the promotion of wound healing might be related to the chemotactic factor, the promotion of angiogenesis and the expression of PCNA and TGF-β1.