| EB病毒活化端粒酶与p16INK4A/E2F1调控的关系 |
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| 引用本文： | 杨静,邓琳,丁琳,柳息洪,曹亚. EB病毒活化端粒酶与p16INK4A/E2F1调控的关系[J]. 现代肿瘤医学, 2003, 11(3): 164-165 |
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| 作者姓名： | 杨静 邓琳 丁琳 柳息洪 曹亚 |
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| 作者单位： | 1. 广州医学院实验医学研究中心,广州,510182
2. 中南大学湘雅医学院肿瘤研究所,湖南,长沙,410078 |
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| 基金项目： | 国家重点基础研究发展规划 (973 )(项目编号:G19980 51201)，国家自然科学基金(项目编号:3 0 200328)，2003年度广东省医学科研基金立项课题(B2 0 0 3 70 ) |
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| 摘 要： | 目的 探讨EB病毒诱导人鼻咽上皮细胞永生化过程中，EB病毒活化端粒酶与EB病毒介导的细胞周期紊乱的关系。方法 利用Western Blotting检测p16^INK4A和E2F1蛋白表达情况；利用hTERT—SEAP报道基因和端粒酶PCR—ELISA分别检测hTERT表达和端粒酶活性。结果 EB病毒下调p16^INK4A表达，并上调E2F1表达；与之相关，EB病毒诱导hTERT表达，并活化端粒酶活性。结论 初步揭示EB病毒通过细胞周期紊乱活化端粒酶活性而诱导人鼻咽上皮细胞永生化的作用。
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| 关 键 词： | EB病毒 端粒酶活化 p16^INK4A 细胞周期紊乱 E2F1 鼻咽癌 |
| 文章编号： | 1672-4992(2003)03-0164-02 |
| 修稿时间： | 2003-02-07 |
Telomerase activation by Epstein-Barr virus is related to p16INK4A/E2F1 regulation |
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| YANG Jing,DENG Lin,DING Lin,et al.. Telomerase activation by Epstein-Barr virus is related to p16INK4A/E2F1 regulation[J]. Journal of Modern Oncology, 2003, 11(3): 164-165 |
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| Authors: | YANG Jing DENG Lin DING Lin et al. |
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| Affiliation: | YANG Jing,DENG Lin,DING Lin,et al. Medical Experimental Center,Guangzhou Medical College,Guangzhou,Guangdong 510182,China. |
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| Abstract: | Objective To study the mechanism of telomerase activation by Epstein-Barr virus in the immortalization of human nasopharyngeal epithelial cells.Methods The expression of p16 INK4A and E2F1 were detected with Western blotting analysis.The hTERT expression and telomerase activity were respectively tested with SEAP report gene assay and with PCR-ELISA analysis in human nasopharyngeal epithelial cells.Results The results showed that p16 INK4A expression was regulated down,at the same time,the expression of E2F1 was up-regulated by Epstein-Barr virus.And both of hTERT expression and telomerase activity increased in EBV-infected cells.Conclusion It is a preliminary study that EBV activated telomerase is through cell cycle to induce human nasopharyngeal epithelial cellular immortalization. |
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| Keywords: | Epstein-Barr virus p16 INK4A E2F1 telomerase activation |
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