带部分松质骨小牛皮质骨支架复合兔骨髓间充质干细胞植入兔体内血管内皮细胞生长因子的表达

摘要 背景：目前异种松质骨作为组织工程材料较为多见，而皮质骨因其难以降解，孔隙率低等原因限制了其使用。但皮质骨具有许多松质骨不具备的优越性，如生物力学方面，如何开发利用皮质骨是课题研究的重点。 目的：观察兔骨髓间充质干细胞复合带部分松质骨小牛皮质骨支架材料植入兔体内后血管内皮细胞生长因子表达。 方法：健康1月龄新西兰大白兔4只用于干细胞提取；3月龄新西兰大白兔60只，在髂骨翼分别植入骨髓间充质干细胞成骨诱导后复合异种骨，单纯异种骨，自体髂骨。术后4，8，12，24周取材RT-PCR检测血管内皮细胞生长因子的表达。 结果与结论：血管内皮细胞生长因子的表达情况：在各时间点，单纯异种骨组低于骨髓间充质干细胞成骨诱导后复合异种骨组和自体髂骨组(P < 0.05)。术后第4周时，骨髓间充质干细胞成骨诱导后复合异种骨组低于自体髂骨组(P < 0.05)，在术后第8，12，24周时，两组差异无显著性意义(P > 0.05)。提示兔骨髓间充质干细胞复合带部分松质骨的小牛皮质骨支架材料植入新西兰兔体内具有较好的血管生成能力。 关键词：成骨诱导；骨髓间充质干细胞；带部分松质骨小牛皮质骨；血管内皮细胞生长因子；骨科生物材料 doi:10.3969/j.issn.1673-8225.2010.34.005

Expression of vascular endothelial growth factor after rabbit bone marrow mesenchymal stem cells combined with calf cortical bone scaffold with partial cancellous bone implanted into rabbits

Abstract BACKGROUND: Currently xenogeneic cancellous bone is more common as tissue engineering materials, but cortical bone is restricted due to degrading difficulty and low porosity. However, cortical bone is inferior to cancellous bone, such as bio-mechanics, how to develop the use of cortical bone is the focus of the research. OBJECTIVE: To observe the expression of vascular endothelial growth factor (VEGF) after rabbit bone marrow mesenchymal stem cells (BMSCs) and cortical bone scaffold with partial cancellous bone were implanted into rabbits. METHODS: Four healthy 1-month-old New Zealand white rabbits were used for stem cell extraction, and 60 3-month-old New Zealand rabbits were implanted with BMSCs induced osteogenic composite calf bone, simple bone xenograft group and autogenous iliac bone into ala of ilium. The expressions of VEGF were detected by RT-PCR at post-operative 4, 8, 12, 24 weeks. RESULTS AND CONCLUSION: The VEGF expression in simple bone xenograft group was lower than that in BMSCs induced osteogenic composite calf bone group and autogenous iliac bone group at all time points (P < 0.05). At postoperative 4 weeks, the BMSCs induced osteogenic composite calf bone group had lower expression than autogenous iliac bone group (P < 0.05), but with no significant differences at postoperative 8, 12, 24 weeks (P < 0.05). Rabbit BMSCs combined with calf cortical bone scaffold with partial cancellous bone implanted into New Zealand rabbits have good angiogenesis capacity.