广东地区TTV感染情况及基因分型

目的 调查广东地区ＴＴ病毒（ＴＴＶ）感染流行情况和基因分型。方法 选取ＴＴＶＯＲＦ１的保守序列作内外引物，Ⅰ型、Ⅱ型同源性极高的序列ｎｔ２４６４～ｎｔ２５２０作包被探针，异源性大于５０％的序列ｎｔ２１２５～ｎｔ２１５９作显色探针Ⅰ和显色探针Ⅱ，采用微板核酸杂交－ＥＬＩＳＡ技术对２８０例肝功能损害的肝炎患者血清进行ＴＴＶ检测及ＴＴＶ基因分型研究。结果 检测出４４例ＴＴＶ阳性患者，检出率为１５．７１％

Study on the prevalence and genotypes of TT virus Infection in Guangdong China

Objective To study the prevalence and genotypes of TT virus(TTV)infection in Guangdong China,Methods Two pairs of primers derived from open reading frame 1(ORF1)of the TT virus genome,capture probe from nt2464～nt2520,a conserved region of the TTV genome,and detector probe from nt2125～nt2159 were used to detect 280 sera samples of hepatitis patients by Microplate Sandwich Hybridization-ELISA technique.Results Forty foLlr(15,71%)of 280 hepatitis patients were positive for TTV DNA.68.18%of which showed genotype Ⅰ infections,9.09%showed genotype Ⅱ infections and 27.73%were mixed genotype Ⅰ/Ⅱ infections.There were 28 chronic hepatitis patients and 16 acute hepatitis patients in 44 TTV positive cases.Conclusion In GLlangdong China.there was higher prevalence of TT virus infection in hepatitis patients,especially chronic hepatitis patients.There were two genotypes of TTV:68.18%of genotype Ⅰ and 9.09%of genotype Ⅱ.Microplate sandwich hybridization-ELISA technique is a sensitive and specific method in studying the prevalene and genotypes of TT virus.