不同人群隐匿性乙型肝炎病毒感染检测分析

目的：探讨不同人群隐匿性乙型肝炎病毒（HBV）感染情况及ELISA检测血清HBsAg阴性的原因。方法：采用巢式聚合酶链反应（PCR）检测150例健康献血员、263例健康体检自然人群和21例不明原因肝炎患者血清HBV DNA。用电化学发光免疫技术对检测出的隐匿性HBV感染者血清HBsAg进行定量测定。结果：隐匿性乙型肝炎病毒感染率分别为1．3％、4．2％和38．1％。电化学发光免疫技术检测隐匿性HBV感染者血清HBsAg，未检出有5例，在0．05ng／ml～0．1ng／ml有7例，0．1ng/ml～0．5ng／ml有6例，0．5ng/ml～10ng/ml有2例。结论：隐匿性HBV感染在不同人群中广泛存在。血清HBsAg阴性的机制主要是HBV复制和基因表达的严重抑制，以及HBV基因突变。

Detection and Analysis on Occult Hepatitis B Virus Infection in Various Populations

Objective： To explore the infection rate of occult hepatitis B virus in various populations and the causes of HBsAg by ELISA. Methods： HBV DNA were determined in the sera from 150 blood donors, 263 general healthy individuals and 21 cryptogenic hepatitis by nested- polymerase chain reaction. Meanwhile, the quantization of HBsAg in the sera from the people with occult hepatitis B virus infection was detected by electro - chemiluminescence immunoassay （ECLIA） , Results： The infection rate of occult hepatitis B virus in these populations was 1.3%, 4.2% and 38.1% respectively. The quantization of HBsAg in the sera from 20 subjects with occult hepatitis B virus infection was： 5 negative, 7 between 0.05ng/ml and 0.1ng/ml, 6 between 0.1ng/ml and 0.5ng/ml, 2 between 0.5ng/ml and 10ng/ml.Conelusion： Occult hepatitis B virus infection exists in various populations, it was related to the existence of anti- HBc closely. Negative for HBsAg in sera from the subjects with occult hepatitis B virus infection were mostly due to a strong suppression of HBV replication and gene expression, HBV gene mutation.