TGFα对胃癌细胞cyclin E和cyclin D1表达的影响

目的 探讨TGFα对胃癌细胞cyclinE和D1表达的促进作用，为研究TGFα促胃癌发生发展机制提供重要信息。方法 ①细胞体外培养，用无血清RPMI-1640阻断法进行G0／G1期同步化，用碘化丙啶染色和流式细胞术，检测其同步化率；②细胞经G0／G1期同步化后，给予2.5％低浓度血清＋RPMI-1640培养处理，以及在此基础上分别加入10、30、50μg／L TGFα处理，均作用5h；用免疫荧光染色及流式细胞术，检测细胞cyclinE和D1的表达情况。结果 细胞经无血清RPMI-1640阻断法处理，其G0／G1期的百分率由常规培养的54％上升到72％；加入TGFα处理后，细胞cyclinE和D1表达均显著高于单用低血清培养（均P〈0.001），并随TGFα剂量的增加而增加；当TGFα浓度为50μg／L时，两者的表达与单用低血清培养比，分别增加了25.18％和27.52％（均P〈0．001）。结论 TGFα可显著促进胃癌细胞cyclinE和D1的表达，从而发挥其促细胞周期进程的作用。

Effect of transforming growth factor α on the expression of cyclin E and cyclin D1 in gastric carcinoma cells

OBJECTIVE: To explore the effect of transforming growth factor alpha (TGFalpha) on the expression of cyclin E and D1 in gastric carcinoma cells. METHODS: Human gastric adenocarcinoma SGC7901 cells were cultured routinely and synchronized at G(0)/G(1) phase in serum-free RPMI-1640. The percentage of the cells at G(0)/G(1) phase was detected by propidium iodide staining and flow cytometry (FCM), and the synchronized cells were cultured in RPMI-1640 supplemented with 2.5% calf serum and treated with 10, 30, and 50 microg/L TGFalpha for 5 h. The expression of cyclin E and D1 in SGC7901 cells was detected by immunofluorescent staining and FCM. RESULTS: The percentage of the cells at G(0)/G(1) phase increased from 54% in routine culture to 72% in the serum-free RPMI-1640 culture. TGFalpha treatment of the cells synchronized at G(0)/G(1) phase induced significant increment of cyclin E and D1 expressions (P<0.001), and at the dose of TGFalpha of 50 microg/L, their expressions increased by 25.18% and 27.52%, respectively (P<0.001). CONCLUSION: TGFalpha can increase the expression of cyclin E and D1 in gastric carcinoma cells to promote their cell cycle progress.