参麦注射液对特发性血小板减少性紫癜患者外周血调节性T细胞的影响

目的：探讨参麦注射液对特发性血小板减少性紫癜患者外周血CD4＋CD2＋5 Treg细胞计数和分泌因子水平的影响。方法选取特发性血小板减少性紫癜患者78例，以随机数字表法分为两组；对照组常规泼尼松治疗，观察组在对照组的基础上加用参麦注射液。对比临床疗效，并分别于治疗前后抽取患者空腹静脉血，以流式细胞法测定CD4＋CD＋25FoxP3＋Treg细胞计数，以ELISA试剂盒测定IL-2、IL-4以及IFN-γ血清含量。结果观察组显效率56．41％、总有效率92．31％，均显著高于对照组的30．77％和71．79％（χ^2＝4．222、3.933，均P ＜0．05）。治疗后观察组和对照组患者外周血 CD4＋CD2＋5 FoxP3＋Treg 细胞计数比30．66％比24.10％、7．49％比5．15％，血清IL-2、IL-4以及IFN-γ血清含量（15．6±4．3） ng／mL比（10．3±3．1） ng／mL、（9．5±2．7）ng／mL比（13．9±3．4）ng／mL、（37．1±4．3）ng／mL比（31．2±4．9）ng／mL，较治疗前均有不等程度的升高，且观察组显著高于对照组（χ^2＝4．329、4．012、4．568、4．105，均P＜0．05）。结论 CD4＋CD2＋5调节性T细胞数量减少或功能缺陷可能参与了特发性血小板减少性紫癜的发病进程，参麦注射液可能能够通过该环节发挥治疗作用。

Effect of Shenmai injection on Treg cells of idiopathic thrombocytopenic purpura

Objective To explore the effect of Shenmai injection on profile and function of CD4＋CD2＋5 Treg cells in ITP patients.Methods 78 ITP patients were selected and divided into the two groups, each group in 39 cases.The control group were given prednisone,while the observation group were given Shenmai injection additionally. CD4＋CD2＋5 FoxP3 ＋Treg cell counts were detected before and after treatment with flow cytometry,and the serum IL-2, IL-4 and IFN-γcontents were detected with ELISA kit.Results The effective rate of 56.41%and total effective rate of 92.31% in the observation group were higher than those of 30.77% and 71.79% in the control group （χ^2 =4.222,3.933,all P〈0.05）.Furthermore,after the treatment,the CD2＋5 FoxP3＋Treg cell counts of 30.66%vs 24.10%and 7.49%vs 5.15%and serum IL-2,IL-4 and IFN-γcontents of （15.6 ±4.3）ng/mL vs （10.3 ±3.1）ng/mL, （9.5 ±2.7）ng/mL vs （13.9 ±3.4）ng/mL,（37.1 ±4.3）ng/mL vs （31.2 ±4.9）ng/mL in both the two groups increased（P〈0.05）;while all indexes′levels of the observation group were higher than those of the control group after treatment（χ^2 =4.329,4.012,4.568,4.105,all P〈0.05）.Conclusion The defeat of CD4＋CD2＋5 Treg cells may be involved in the process of ITP,and Shenmai injection may be able to play a role in treatment through this link.