| FATP4在大鼠非酒精性脂肪肝中的表达及相关性 |
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| 引用本文： | 冯爱娟,陈东风,樊丽琳,胡辂,史洪涛.FATP4在大鼠非酒精性脂肪肝中的表达及相关性[J].重庆医学,2007,36(8):707-708,711. |
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| 作者姓名： | 冯爱娟 陈东风 樊丽琳 胡辂 史洪涛 |
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| 作者单位： | 1. 北京军区总医院263临床部消化内科,101149
2. 第三军医大学大坪医院野战外科研究所消化内科,重庆,400042 |
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| 摘 要： | 目的 探讨FATP4(fatty acid transport protein4)基因在大鼠非酒精性脂肪肝形成中的作用.方法 Wistar大鼠64只,随机分为正常对照组(C组)和高脂饮食组(F组),又各分2、4、8和12周组(其中每组各8只):HE染色光镜观察肝脏组织病理改变;硫代巴比妥酸法测定肝脏组织丙二醛(MDA)的含量变化:免疫组织化学和Western blot方法研究高脂饲料诱导的大鼠脂肪肝形成中FATP4表达变化.结果 高脂饲料组大鼠肝脏内MDA含量明显高于对照组,随时间延长,逐渐增加:随着脂肪肝程度的加重,MDA含量逐渐增强,肝细胞FATP4蛋白表达亦明显增强.结论 非酒精性脂肪肝大鼠肝细胞FATP4表达变化与脂肪肝引起的脂质过氧化损伤程度密切相关.
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| 关 键 词： | 非酒精性脂肪肝 FATP4 免疫组织化学 Western blot 丙二醛 |
| 文章编号： | 1671-8348（2007）08-707-03 |
| 修稿时间： | 2006-12-12 |
Effect of fatty acid transport protein 4 on rat non-alcoholic fatty liver |
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| FENG Ai-juan , CHEN Dong- feng , FAN Li-lin ,et al..Effect of fatty acid transport protein 4 on rat non-alcoholic fatty liver[J].Chongqing Medical Journal,2007,36(8):707-708,711. |
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| Authors: | FENG Ai-juan CHEN Dong- feng FAN Li-lin |
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| Institution: | Department of Gastroenterology, Institute of Field Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China |
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| Abstract: | Objective To study the effect of fatty acid transport protein(FATP4)on the model of rat non-alcoholic fatty liver.Methods Expression of FATP4 antigen in rat nonalcoholic steatosis model induced by high diet was detected with immunehistochemistry and Western blot.Meanwhile,the level of malondialdehyde(MDA) in liver tissue was detected by using thio-barbituric acid.Results The level of MDA in liver tissue and the expression of hepatocyte FATP4 antigen in rat nonalcoholic steatosis model induced by high fat diet were significantly increased compared with normal group.There was significantly positive correlation between MDA,FATP4 expression and degree of nonalcoholic steatosis.Conclusion Nonalcoholic steatosis induced liver damage is associated with a generalized induction of hepatocyte FATP4 and lipid peroxidation. |
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| Keywords: | non-alcoholic fatty liver FATP4 immunohisochemistry MDA Western blot |
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