| 杂交捕获法和PCR-RDB法检测妇女HPV感染的比较 |
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| 引用本文： | 肖克林,吴丽娟,王敏,郑有为,钱靖琳,王丁,周克元. 杂交捕获法和PCR-RDB法检测妇女HPV感染的比较[J]. 国际检验医学杂志, 2007, 30(1): 545-547. DOI: 10.3760/cma.j.issn.1673-4130.2009.06.009 |
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| 作者姓名： | 肖克林 吴丽娟 王敏 郑有为 钱靖琳 王丁 周克元 |
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| 作者单位： | 深圳市宝安区妇幼保健院检验科,518133;中山大学达安基因股份有限公司科研部,广州,510665;广东省人民医院病理医学部,广州,510080;广东医学院生物化学与分子生物学教研室,广东湛江,524023; |
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| 摘 要： | Objective To compare hybrid capture-Ⅱ(HC2)to polymerase chain reaction(PCR)-reverse dot-blot(RDB)for detecting HPV infection in women.Methods HC2 method and PCR-RDB were applied to detecting human papillomaviral infection in female genital duct,and the test results were analyzed.Results Concordant results were found in 81.14%(400/493)samples(Kappa=0.63;95%CI,0.57-0.69).The high risk type HPV positive rate detected by PCR-RDB were 1.60%(3/187),29.85%(20/67),69.88%(58/83),86.52%(77/89)and 91.04%(61/67),respectively,when the HC2 RLU/CO of samples were＜1.00,1.00-9.99,10.00-99.99,100.00-999.99 and≥1000.00.Statistical differences were found among the five groups(χ2=289.3,P＜0.01).Cross reactions were found between HC2 high risk probe and HPV53,66,11,cp8304 and other genotypes.Conclusion There is good concordance between HC2 and PCR-RDB,though cross reaction exists in HC2.Relatively speaking,there are more influential factors for PCR-RDB.
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| 关 键 词： | 人乳头瘤病毒16 人乳头瘤病毒18 聚合酶链反应 交叉反应 |
Comparison between HC2 and PCR-RDB for detecting HPV infection in women |
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| Keywords: | Human papillomavirus 16Human papillomavirus 18Polymerase chain reactionCross reactions |
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