氨甲喋呤对映体对人肝癌细胞HepG-2增殖的抑制作用

目的]研究手性药物氨甲喋呤（MTX）单一对映体对人肝癌细胞株HepG-2的体外增殖的抑制作用。方法]应用MTT比色法检测MTX对映体（＋）MTX、（-）MTX]对HepG-2细胞增殖抑制作用,用倒置显微镜观察细胞的形态学变化。结果]0.1～150μmol/L（＋）MTX和10～150μmol/L（-）MTX可抑制HepG-2细胞的生长,且其作用具有剂量和时间依赖性（P〈0.01）,但抑制强度为（＋）MTX〉（-）MTX;倒置显微镜观察不同浓度（＋）MTX和（-）MTX作用HepG-2细胞株不同时间后,细胞出现不同程度的形态学改变。结论]（＋）MTX和（-）MTX对HepG-2细胞的抑制作用不同,（＋）MTX抗增殖作用明显强于（-）MTX。

The Inhibition of Methotrexate Enantiomers on Human Hepatoma HepG-2 Cell Proliferation

Purpose] To study the inhibition of methotrexate（MTX） enantiomers on proliferation of human Hepatoma HepG-2 cell line. Methods] The inhibition of MTX enantiomers （＋）MTX, （-）MTX] on proliferation of HepG-2 cells were detected by the MTT assay. The morphological profile were examined by inverted microscopy. Results] Both （＋）MTX dosed 0.1-150μmol/L and （-）MTX dosed 10-150μmol/L inhibited the growth of HepG-2 cells in a time- and dose-dependent manner （P〈0.01）.The inhibition of （＋）MTX was stronger than that of （-） MTX. When the different concentration of MTX enantiomers were administrated at the different times, the different morphologies of HepG-2 cells were found. Conclusions] The inhibition of （＋）MTX and （-）MTX on HepG-2 cells was different and the anti-proliferation of （＋）MTX was significantly stronger than that of （-）MTX.