银屑病患者皮损中Toll样受体2及相关因子的检测

目的探讨Toll样受体2(TLR2)、信号途径下游分子NF-κBp65以及可能的效应分子TGF-α在银屑病患者皮损中的表达及其与银屑病严重程度的关系。方法采用EliVision免疫组化法对40例银屑病患者进行期皮损、11例非皮损区及15例正常人皮肤中TLR2,NF-κBp65,TGF-α的表达进行检测,对其在皮损中的表达进行相关性分析,并将结果与PASI评分进行相关性分析。结果与正常人皮肤及银屑病患者非皮损区相比,银屑病患者皮损表皮中TLR2,NF-κBp65,TGF-α的表达明显上调,而非皮损区TLR2的表达也高于正常人皮肤,差异均有统计学意义(P<0.05)。银屑病患者皮损表皮中TLR2,NF-κBp65的表达水平与PASI评分之间存在正相关(P<0.05)。银屑病患者皮损表皮中TLR2与NF-κBp65,TLR2与TGF-α,NF-κBp65与TGF-α表达水平之间均存在正相关(P<0.05)。结论TLR2,NF-κBp65,TGF-α在银屑病中表达异常,可能共同参与了银屑病的发病过程。

Determination of Toll-like Receptor 2 and Its Related Factors Expression in Psoriatic Lesions

Objective To investigate the expressions of Toll-like receptor 2(TLR2),nuclear-factor-κB(NF-κB)p65 and transforming growth factor-alpha(TGF-alpha)in the epidemis of psoriasis and their correlation with disease severity in psoriatic patients.Methods Forty samples of lesional psoriatic skin,eleven samples of nonlesional psoriatic skin and fifteen samples of normal skin were analyzed by EliVision immunohistochemical technique for the expressions of TLR2,NF-κB p65 and TGF-alpha protein.Correlation analysis was performed among the expressions of three factors as well as between the expressions and disease severity.Results Compared with that of normal epidermis and nonlesional psoriatic epidermis,TLR2,NF-κB p65 and TGF-alpha were more highly expressed in lesional epidermis from patients with psoriasis(all P<0.05).In nonlesional psoriatic epidermis,TLR2 expression was also upregulated compared with that of normal epidermis(P<0.05).The expressions of TLR2 and NF-κB p65 in lesional epidermis positively correlated individually with the psoriasis area and severity index(PASI)scores in patients(both P<0.05).Positive correlation was also found between the expressions of any two factors(TLR2,NF-κB p65 or TGF-alpha)in lesional epidermis(all P<0.05).Conclusion The upregulation of TLR2,NF-κB p65 and TGF-alpha expressions in psoriatic lesions may be involved in the pathogenesis of psoriasis.