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Low-level laser therapy combined with platelet-rich plasma on the healing calcaneal tendon: a histological study in a rat model |
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| Authors: | Danillo Barbosa Renato Aparecido de Souza Wellington Roberto Gomes de Carvalho Murilo Xavier Paula Kariluce de Carvalho Tereza Cristina Rodrigues Cunha Emilia Ângela Loschiavo Arisawa Landulfo Silveira Jr Antonio Balbin Villaverde |
| Affiliation: | 1. Biomedical Engineering Center, Camilo Castelo Branco University (UNICASTELO), Estrada Dr. Altino Bondesan, 500, Distrito de Eugênio de Melo, 12247-004, S?o José dos Campos, S?o Paulo, Brazil 2. Faculdade de Odontologia, Instituto Nacional de Ensino Superior e Pós Gradua??o Padre Gervásio (INAPOS), Rua Jo?o Basílio, 219, 37550-000, Pouso Alegre, Minas Gerais, Brazil 3. Av. Shishima Hifumi, 2911, 12244-000, S?o José dos Campos, S?o Paulo, Brazil 5. Grupo de Estudos e Pesquisa em Ciências da Saúde (GEP-CS), Instituto Federal de Educa??o, Ciência e Tecnologia do Sul de Minas Gerais (IFSULDEMINAS), Rua Dinah, 75, 37890-000, Muzambinho, Minas Gerais, Brazil 6. Center for Biological and Health Sciences, Department of Physical Education, Federal University of Maranh?o, Avenida dos Portugueses, S/N Bacanga, 65085-580, Sao Luis, MA, Brazil 7. Department of Physiotherapy, Federal University of Jequitinhonha and Mucuri Valleys (UFVJM), Rodovia MGT 367-Km 583, no. 5000, 39100-000, Diamantina, Minas Gerais, Brazil 4. Institute of Research and Development (IP&D), Vale do Paraíba University (UNIVAP), Av. Shishima Hifumi, 2911, 12244-000, S?o José dos Campos, S?o Paulo, Brazil |
| Abstract: | The objective of this study was to investigate the effects of low-level laser therapy (LLLT) treatment alone (λ?=?660 nm and λ?=?830 nm) or associated with platelet-rich plasma (PRP). We used 54 male rats divided into six groups, with nine animals each: group 1, partial tenotomy; group 2 (GII), PRP; group 3 (GIII): λ660?nm; group 4 (GIV), λ830?nm; group 5 (GV), PRP + λ660?nm; and group 6 (GVI), PRP + λ830?nm. The protocol used was power density 0.35 W/cm2, energy 0.2 J, energy density 7.0 J/cm2, time 20 s per irradiated point, and number of points 3. Animals in groups GII, GV, and GVI received treatment with PRP, consisting of a single dose of 0.2 mL directly into the surgical site, on top of the tenotomy. Animals were killed on the 13th day post-tenotomy and their tendons were surgically removed for a quantitative analysis using polarization microscopy. The percentages of collagen fibers of types I and III were expressed as mean ± SD. Higher values of collagen fibers type I were obtained for groups GV and GVI when compared with all other groups (p?p?>?0.05). For collagen type III, a significant difference was observed between GII and all other groups (p? |
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