两种工艺制备的参芪扶正注射液对荷S180小鼠细胞免疫功能的影响

目的:比较两种工艺制备的参芪扶正注射液对荷S₁₈₀小鼠细胞免疫功能的影响。方法:将42只荷瘤小鼠随机分成5组,分别为对照组、现工艺制备的参芪扶正注射液高剂量组(8g·kg^-1)和临床等效剂量组(4g·kg^-1)、原工艺制备的参芪扶正注射液高剂量组(8g·kg^-1)和临床等效剂量组(4g·kg^-1)。腹腔注射给药,1次/d,实验组给予不同剂量的相应参芪扶正注射液,对照组给予生理盐水。7d后从小鼠眼眶后静脉丛取血进行全血细胞检测及T淋巴细胞亚群检测。结果:4个实验组白细胞和淋巴细胞总数皆高于对照组,其中现工艺4g·kg^-1组与对照组的差异有统计学意义(P<0.01);4g·kg^-1剂量组中,现工艺淋巴细胞总数显著高于原工艺(P<0.05);现工艺4g·kg^-1组的CD4+淋巴细胞比例显著高于对照组(P<0.05);同一剂量两种工艺制剂之间T淋巴细胞亚群比例无显著差异。结论:在临床等效剂量上,现工艺制备的参芪扶正注射液可提高荷S₁₈₀小鼠白细胞、淋巴细胞及CD4⁺T细胞的水平,效果优于或等于原工艺。

Effects of Shenqi Fuzheng Injections Prepared by Two Different Crafts on the Cellular Immune Function in S180-bearing Mice

Objective:To compare effects of Shenqi Fuzheng Injections prepared by two different crafts on the cellular immune function in S₁₈₀-bearing Mice.Methods: 42 tumor-bearing mice randomly divided into five groups:the model group,the high dose group(8 g·kg^-1) and the clinical equivalent dose group(4 g·kg^-1) of Shenqi Fuzheng Injection on current craft,the high dose group(8 g·kg^-1) and the clinical equivalent dose group(4 g·kg^-1) of Shenqi Fuzheng injection on previous craft.The model group was given physiological saline by intraperitoneal injection once a day,while the other groups was given different Shenqi Fuzheng Injections in the same way.7 days later,blood sample was taken from the vena orbitalis posterior of the mice and blood count and the subpopulation of T lymphocyte were determined.Results: The counts of the white blood cell and lymphocyte in four Shenqi Fuzheng Injection groups were higher than the model group,and the difference between the 4 g·kg^-1 group of Shenqi Fuzheng Injection on current craft and the model group was very significant(P<0.01),and in the 4 g·kg^-1 groups,the count of lymphocyte in the current craft group was significantly higher than the previous one(P<0.05).The ratio of CD4⁺ T lymphocyte in the 4 g·kg^-1 group on current craft was significantly higher than the model one(P<0.05).There was no significant difference between the ratio of the subpopulation of T lymphocyte on the current craft and the previous one at the same dose.Conclusion: In clinical equivalent dose(4 g·kg^-1),the Shenqi Fuzheng Injection prepared by the current craft could enhance the counts of the white blood cell and lymphocyte and the radio of CD4⁺ T lymphocyte,and the effectiveness was better than or equal to the previous craft.