| 电损伤骨骼肌细胞微区Ca^2＋的实验研究 |
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| 引用本文： | 黄志斌,汪道新,凌云志,唐亚飞,张力勇,朱志祥.电损伤骨骼肌细胞微区Ca^2＋的实验研究[J].海南医学,2009,20(4):113-115. |
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| 作者姓名： | 黄志斌 汪道新 凌云志 唐亚飞 张力勇 朱志祥 |
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| 作者单位： | 1. 广东省惠州市惠阳人民医院烧伤整形科,广东,惠州,516211
2. 广东省深圳市第二人民医院烧伤整形科,广东,深圳 |
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| 摘 要： | 目的观察电损伤骨骼肌细胞微区Ca2＋变化,探讨它在电损伤中的作用。方法新西兰大白兔36只,随机均分为实验组和对照组,选取其右后肢胫骨前肌为研究对象。以电压600V,电击时间9ms,累积电击3次,造成单束骨骼肌电损伤。分别于处理后2h、8h、24h、48h、72h、5d每组随机抽取3只兔,留取肌组织标本。EPMA法检测肌细胞微区Ca^2＋含量。结果实验组细胞浆Ca^2＋快速升高,肌浆网Ca2＋降低,而线粒体Ca^2＋含量8h才明显上升,三微区Ca^2＋含量至5d仍未恢复正常水平,与对照组比较差异有统计学意义。实验组细胞浆和肌浆网Ca^2＋呈反向性变化,细胞浆和线粒体则呈同向性变化。结论电击后骨骼肌细胞三微区Ca^2＋发生再分布,并存在长时间的细胞钙超载,它可能是电损伤骨骼肌进行性坏死的重要原因。
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| 关 键 词： | 骨骼肌 电损伤 钙离子 |
Experimental study on subcellular Ca~(2+) of electric injuried skeletal muscles |
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| HUANG Zhibin,WANG Daoxin,LING Yunzhi,et al..Experimental study on subcellular Ca~(2+) of electric injuried skeletal muscles[J].Hainan Medical Journal,2009,20(4):113-115. |
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| Authors: | HUANG Zhibin WANG Daoxin LING Yunzhi |
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| Institution: | HUANG Zhibin,WANG Daoxin,LING Yunzhi,et al.Plastic surgery & Burns Department,Huiyang People's Hospital,Huiyang 516211,Guangdong,P.R.CHINA |
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| Abstract: | Objective To observe the changes of subcellular Ca2+ of electric injuried skeletal muscles.Methods The right rear anterior tibial muscles of 36 New Zealand rabbits which are divided equally into the control group and experimental group,was stroked on 600 voltage,9ms shock duration and 3 cumulative times.The specimens,took at 2h,8h,24h,48h,72h and 5d postinjury,were detected subcellular(included cytoplasm,mitochondria,sarcoplasm reticulum)Ca2+ contents by way of EPMA.Results In experimental group,the content... |
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| Keywords: | Skeletal muscle Electrical injuries Calcium ion |
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