三种中药复方对脑缺血再灌注大鼠血浆血管性血友病因子和血栓调节蛋白影响的比较研究

目的比较三种中药复方对脑缺血再灌注大鼠血浆血管性血友病因子（vWF）和血栓调节蛋白（TM）动态的影响。方法采用线栓法制作大鼠局灶性脑缺血再灌注模型，随机分为假手术组、模型组、益气活血方组、镇肝息风汤组和星蒌承气汤组，分别给予相应药物灌胃；采用酶联免疫ELISA法检测血浆vWF和TM含量。结果模型组再灌注各时间点血浆vWF和TM含量显著升高；再灌注各时间点各治疗组vWF和TM含量显著降低；24h时在降低vWF方面以镇肝息风汤组为优，在降低TM方面以星蒌承气汤组为优；48h时在降低TM方面以星蒌承气汤和益气活血方组为优，在降低vWF方面以星蒌承气汤组为优；72h时均以益气活血方组为优。结论三种中药复方可以通过降低vWF和TM含量抗脑缺血再灌注损伤，72h益气活血方作用效果最佳。

Comparative Study on Effects of Three Traditional Medicinal Compounds on vWF and TM in Plasma of Rats after Focal Cerebral lschemia and Reperfusion

Objective： To compare the effects of three traditional Chinese medicinal compounds on vWF and TM in plasma of rats after focal cerebral ischemia and reperfusion（I/R）. Methods： The local cerebral I/R model was established by ligation of the middle cerebral arteries. The animals were divided into the sham- operative group, the model group, the Yiqihuoxue Recipe（YHR） group, the Zhengganxifeng Decoction（ZXD） group and the Xin- glouchengqi Decoction （XCD）group. The levels of vWF and TM were measured by enzyme-linked immunosorbent in plasma. Results： The levels of vWF and TM were higher in model group than those in sham - operative group at all time points after I/R. Compared with those in model group, the levels of vWF and TM were lower in all treatment groups. The optimal effect was shown in ZXD group（vWF） or in XCD group（TM） at 24h, in XCD group（vWF） or in XCD group and YHR group （TM） at 48h, and in YHR group at 72h after I/R. Conclusion： The three traditional Chinese medicinal compounds reduce pathologic injury after focal cerebral I/R in rats by inhibiting levels of vWF and TM in plasma , the optimal effect of YHR was shown at 72h after I/R.