组合LC-MS-MS鉴定技术的生物活性导向研究合欢皮抗炎活性部位

目的：寻找合欢皮的抗炎活性部位。方法：以巴豆油引起小鼠耳肿胀模型做为抗炎筛选模型,同时,在LC-MS-MS分析的导向下,寻找合欢皮抗炎活性部位。结果：合欢皮乙醇提取物的正丁醇萃取部位(AJ-B)是主要活性部位。该部位进一步分离后的木脂素苷类部位(AJ-B-1)显示出明显的抗炎活性,并在5～20 mg·kg^-1时表现出一定的剂量依赖关系。结论：LC-MS-MS导向与活性评价导向分离相结合,有助于较快和更理性地进行中药活性部位和成分的研究。

Studies on bioassay-guided anti-inflammatory fraction in bark of Albizia julibrissin combined determination with LC-MS-MS

Objective: To search the anti-inflammatory fraction of Albizia julibrissin. Method: Inflammatory model of Kunming mice ear edema induced by croton oil and determination combined with the LC-MS-MS-guided fractionation and isolation were used. Result: The n-butanol fraction (AJ-B) obtained from the ethanolic extract of the Cortex albiziae was the major active fraction. The lignan glycosides fraction (AJ-B-1),which was further isolated from AJ-B, showed significant anti-inflammatory activity and exhibited dose-dependent relationship in the dose of 5 to 20 mg·kg^-1. Conclusion: The method of bioassay-guided fractionation and isolation combined with the LC-MS-MS determination may be of benefit to the logical studies on the bioactive fractions or constituents of traditional Chinese materia medica.