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Identification of β-aminoisobutyric acid in uremic serum |
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| Authors: | Fumitake Gejyo Yasutami Kinoshita Tokuji Ikenaka |
| Affiliation: | a Department of Internal Medicine, Niigata University School of Medicine,., Niigata 951, Japan b Department of Biochemistry, Niigata University School of Medicine,., Niigata 951, Japan |
| Abstract: | An unidentified ninhydrin-positive substance found in uremic sera but not found in normal sera was isolated by gel-filtration through Sephadex G-75 followed by high voltage paper electrophoresis (pH 3.5), and identified as β-aminoisobutyric acid using paper chromatography and automated amino acid analyzer. The quantitative determination of β-aminoisobutyric acid in serum revealed that the level of β-aminoisobutyric acid in uremic sera was much higher than that of normal sera. Gas chromatographic determination of the enantiomorphs of β-aminoisobutyric acid showed that uremic sera contain R- and S-isomers of the amino acid, but with the R-isomer as the dominating form. |
| Keywords: | Reprint requests should be sent to: Dr. F. Gejyo 2nd Department of Internal Medicine Niigata University School of Medicine 757 Asahimachi-Dori 1-Bancho Niigata 951 Japan. |
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