单细胞凝胶电泳法检测二硫化碳染毒小鼠精子DNA损伤

目的　用碱性单细胞凝胶电泳技术检测CS2 染毒小鼠精子DNA损伤 ,研究CS2 的遗传毒性。方法　用单细胞凝胶电泳法 ,以DNA断裂分级及DNA彗星尾长和尾矩来评价CS2 对精子DNA的损伤。结果　CS2 染毒剂量组均出现DNA彗星的拖尾率增加 (分别为 6 7.14 %、84 .2 9%和 91.0 0 % )、损伤强度指数增高 (分别为 5 0 7、6 5 6和 74 5 )、DNA头部百分比减少 (分别为 84 .5 5 %、73.84 %和5 5 .71% )、彗星尾长 (分别为 5 .87、8.81、13.4 9μm)及尾矩 (分别为 1.30、1.6 3、2 .6 6 μm)增加 ,与阴性对照组比较 ,差异均有显著性 (P <0 .0 5 )。结论　单细胞凝胶电泳能够快速、敏感地检测CS2 染毒小鼠精子DNA损伤 ,该方法可用于环境致癌物和致突变物的检测。

Detection of DNA damage induced by carbon disulfide in mice sperm with single-cell gel electrophoresis assay

OBJECTIVE: To study the genotoxicity of carbon disulfide by detecting DNA damage in mice sperm with single-cell gel electrophoresis assay (SCGE). METHODS: SCGE was used to detect sperm DNA damage. The index of DNA damage, tail length and tail moment were used to evaluate the extent of DNA damage. RESULTS: In three dosage groups, the rate of DNA damage (67.14%, 84.29% and 91.00%, respectively), index of DNA damage intensity (507, 656 and 745, respectively), tail length (5.87, 8.81 and 13.49 microm, respectively) and tail moment (1.30, 1.63, 2.66 microm, respectively) were significantly increased, while the percentage of head of the comet was significantly decreased (84.55%, 73.84% and 55.71%, respectively). A significant changes were clearly observed in all dosage groups compared to those of the control group (P<0.05). CONCLUSION: SCGE which is a quick and sensitive method to detect DNA damage induced by CS2 may be used to monitor carcinogen and mutagen.