人肝转移结直肠癌微小核糖核酸表达谱的初步研究

目的 研究人肝转移结直肠癌、无肝转移结直肠癌中微小核糖核酸(miRNA)的表达谱,筛选与结直肠癌肝转移相关的miRNA.方法 收集25例手术切除结直肠癌标本液氮储存.分别选取3例无肝转移和3例肝转移结直肠癌组织,提取组织总RNA,采用illumina miRNA芯片技术检测两种组织中miRNA的表达,筛选两种不同组织中差异表达miRNA,采用实时定量PCR技术在全部结直肠癌组织中对芯片检测结果进行验证.结果 肝转移结直肠癌组织与无肝转移结直肠癌组织中miRNA表达有明显差异,与无肝转移结直肠癌组织相比,在肝转移结直肠癌组织中差异表达的miRNA有28个,其中4个上调,24个下调.肝转移结直肠癌组织中miR-139-3p表达量为1.75±0.40,较无肝转移上调(0.69±0.58,P<0.05),而miR-19a在肝转移中的表达量为0.39±0.20,较无肝转移下调(1.38±0.98,P<0.05),miRNA芯片结果与定量RT-PCR结果一致.结论 miRNA的异常表达可能与结直肠癌的肝转移有关,特异的miRNA表达谱可以为结直肠癌的肝转移提供新的诊断和治疗靶点.

The initial study on microRNA expression profile in human colorectal cancer with liver metastasis

Objective To investigate the microRNAs expression profile in human colorectal cancer with or without liver metastasis and try to screen miRNA associated with liver metastasis in colorectal cancer. Methods Twenty five surgical resected colorectal cancer specimens were collected and frozen in liquid nitrogen. Three without liver metastasis and three with liver metastasis were selected, from which total RNAs were isolated. The expressions of miRNAs in these two types of specimens were detected by illumine microRNA microarray, and the difference of miRNA expression was screened. The biochip results were verified with real-time RT-PCR in all colorectal caner specimens. Results The miRNA expression was significantly different in colorectal cancer with liver metastasis and without liver metastasis. Compared with colorectal cancer without liver metastasis, 28 miRNA expressions was different in colorectal cancer with liver metastasis, 4 up regulated and 24 down regulated. The quantity of miR-139-3p expression in colorectal cancer with liver metastasis was 1.75±0.40, up regulated compared with that incolorectal cancer without liver metastasis(0. 69 ±0.58,P＜0.05). The quantity of miR-19a expression in liver metastasis was 0. 39±0. 20, downregulated compare with no liver metastasis( 1.38 ± 0.98, P＜0. 05). The result of miRNA biochip was consistent with that of RT-PCR. Conclusion The difference of miRNA expression might relate to liver metastasis of colorectal cancer. The specific miRNAs expression profile might provide new target for diagnosis and treatment of colorectal cancer with liver metastasis.