| 1,25-(OH)_2D_3-26,23-Lactone的一步高压液相色谱纯化法 |
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| 引用本文： | 郑明慈,高莉丽.1,25-(OH)_2D_3-26,23-Lactone的一步高压液相色谱纯化法[J].营养学报,1993(1). |
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| 作者姓名： | 郑明慈 高莉丽 |
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| 作者单位： | 桂林医学院附院儿科，桂林医学院附院儿科 桂林 541001，桂林 541001 |
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| 摘 要： | 用顺相HPLC一次性纯化1,25-二羟胆骨化醇类脂1,25-(OH)_2D_3-26,23-Lactone],流动相为:甲醇、二氯甲烷、正己烷的混合液。流速为2ml/min。1,25-二羟胆骨化醇(1,25-(OH)_2D_3)的保留时间是12分10秒,1,25-(OH_2)D_3-26,23-Lactone的保留时间是17分6秒,1,24,25-三羟胆骨化醇1,24,25-(OH)_3D_3]的保留时间是22分。该法能用HPLC一次性分离出1,25-(OH)_2D_3;1,25-(OH)_2-D_3-26,23-Lactone和1,24,25-(OH)_3D_3。具有快速、准确、节约试剂的特点。
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| 关 键 词： | 顺相HPLC 1 25-(OH)_2D_3-26 23-Lactone 纯化法 |
STUDY ON METHOD OF PURIFYING 1,25-(OH)_2D_3- 26,23-LACTONE WITH ONE STEP OF HPLC |
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| Zheng Minci,Gao Lili.STUDY ON METHOD OF PURIFYING 1,25-(OH)_2D_3- 26,23-LACTONE WITH ONE STEP OF HPLC[J].Acta Nutrimenta Sinica,1993(1). |
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| Authors: | Zheng Minci Gao Lili |
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| Abstract: | An improved method for the purification of 1,25-(OH)2D3-26,23-lactone was established by using one step of highperformance liquid chromatography (HPLC). The analytic column was Shim-pack NH2 (straight-phase type). The mobile phase was Methanol: Dichloromethane: Hexane = 4:26:70 and the flow rate was 2ml/min. An ultraviolet detector with 254nm was used. The retention time of 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1, 24, 25-(OH)3D3 werel2.10, 17.06 and 22,00 minutes respectively. This method can separate 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1,24,25-(OH)3D3 only by one step of HPLC and it is better than the old one which need two steps of HPLC for the separation of these substances. |
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| Keywords: | HPLC straight-phase type column 1 25-(OH)2D3-26 23-lactone |
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