茅台酒对大鼠肝组织中CYP2E1基因及蛋白质表达的影响

目的：探讨茅台酒对大鼠肝组织中细胞色素P4502El（CYP2E1）基因及蛋白质表达的影响。方法：40只雄性Wistar大鼠随机分为空白对照组、茅台酒组、乙醇组，各组大鼠分别予蒸馏水、茅台酒和乙醇灌胃，每天1次，每周6次，连续12周。末次灌胃12h后处死大鼠，肝组织切片行HE染色观测病理改变，并检测肝组织匀浆中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）与丙二醛（MDA）含量，RT—PCR、免疫组织化学法和WesternBlot检测大鼠肝组织中CYP2E1mRNA及蛋白质表达。结果：茅台酒组及乙醇组大鼠肝组织切片HE染色均可见不同程度肝细胞脂肪变性及炎性细胞浸润，但茅台酒组的病理改变程度明显低于乙醇组，差异有统计学意义（P〈0．05）；茅台酒组大鼠肝组织匀浆中SOD、GSH—P。比空白对照组和乙醇组高，差异有统计学意义（P〈0．01），与乙醇组比，茅台酒组大鼠肝组织匀浆中MDA显著较低（P〈0．01）；茅台酒组与空白对照组比较，大鼠肝组织中CYP2E1mRNA及蛋白质表达差异无统计学意义（P〉0．05），茅台酒组与乙醇组比较＋大鼠肝组织CYP2E1mRNA及蛋白质表达显著较低（P〈0．01）。结论：饮用一定量的茅台酒12周后可致大鼠酒精性脂肪肝，但比饮用相同剂量单纯乙醇程度较轻，可能与茅台酒所含的非酒精性物质抑制了CYP2E1表达，对抗氧化应激有关。

Effect of Maotai Liquor on CYP2E1 Expression of Hepatic Tissues of Rats

Objective： To explore the influence of Maotai liquor on the expression of cytochrome P450 2El （CYP2E1） in liver tissue of rats suffered from alcohol. Methods： Forty male Wistar rats were divided into 3 groups and were intragastrically administered with 10 mL/kg distilled water （con- trol group）, 10 mL/kg Maotai liquor contained 53% ethanol （Maotai liquor group）, and 53% alcohol solution （ethanol group） once a day, 5 days per week, and lasting for 12 weeks. Hepatic pathology was assessed by HE stain. Superoxide Dismutase （SOD）, glutathione peroxidase （GSH-PX） and Ma- londialdehyde （MDA） in liver tissue homogenate were determined by assay kits. Real-time PCR, im- munohistochemistry and Western blot were used to determine expression levels of CYP2E1 in liver. Results： Hepatic fatty degeneration and inflammatory liquor group and ethanol group, which were infrequent cells infiltration were observed in both Maotai in Maotai liquor group relatively （ P 〈 0.05 ）. Compared with the control group and ethanol group, levels of SOD and GSH-PX in liver tissue homoge- nate in Maotai liquor group were higher significantly（P 〈0.01 ）, meanwhile the level of MDA in Mao-tai liquor group were lower significantly compared with ethanol group （P 〈 0.01 ） ; Compared with etha- nol group, levels of mRNA and protein of CYP2E1 in Maotai liquor group were lower significantly （P 〈0.01 ）. Conclusions： Maotai liquor consumption （10 mL/kg） for 12 weeks may bring about alco- holic fatty liver in rats, but the degree is lower than that induced by pure ethanol. It perhaps is on ac- count of the inhibition of CYP2E1 expression by non-ethanol components of Maotai liquor.