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Effect of 5-Aminoisoquinolinone on the Adhesion of Colon Carcinoma
引用本文:王娅兰,郝兰香. Effect of 5-Aminoisoquinolinone on the Adhesion of Colon Carcinoma[J]. 中国癌症研究, 2007, 19(2): 119-123. DOI: 10.1007/s11670-007-0119-0
作者姓名:王娅兰  郝兰香
作者单位:Department of Pathology, Chongqing Medical University, Chongqing 400016
摘    要:Objective: 5-Aminoisoquinolinone, a water-soluble, potent inhibitor of the activity of poly (adenosine 5'-diphosphate ribose) polymerase, plays an important role in the tissue injury associated with ischaemia-reperfusion injury and inflammation by inhibiting the activity of poly (adenosine 5'-diphosphate ribose) polymerase and the expression of cell adhesion molecules such as ICAM-1, P-selectin et al. But how about it in the tumor is not clear. The aim of the present study was to study the effects of 5-Aminoisoquinolinon on the adhesion of colon carcinoma line HT-29 cells to human umbilical vein endothelial cells; and the effects of 5-Aminoisoquinolinon on the expression of ICAM-1, P-selectin and the activity of poly (adenosine 5'-diphosphate ribose) polymerase in colon carcinoma HT-29 cells. Methods: The adhesion of HT-29 cells to human umbilical vein endothelial cells was detected by adhesive experiment. Immunocytochemically Streptavidin-Peroxidase method was used to investigate the expression of ICAM-1, P-selectin and Poly (adenosine 5'-diphosphate ribose)( the product of poly (adenosine 5'-diphosphate ribose) polymerase activation). Results: the results of the adhesion assay of HT-29 cells to HUVEC showed that the OD570 value in each 5-AIQ-treated group was significant lower than that in the control group (5-AIQ-untreated) in a dose-dependent manner. The expression of ICAM-1, P-selectin and Poly (adenosine 5'-diphosphate ribose) was significant lower in 5-Aminoisoquinolinone-treated HT-29 cell group than that in 5-Aminoisoquinolinoneuntreated groups. Conclusion: The data suggest that 5-Aminoisoquinolinone can inhibit the adhesion of HT-29 cells to human umbilical vein endothelial cells. 5-Aminoisoquinolinone also can inhibit poly (adenosine 5'-diphosphate ribose) polymerase activation and the expressions of ICAM-1 and P-selectin in HT-29 cells. 5-Aminoisoquinolinone probably contributes to the prevention of tumor cell metastasis. Further study is needed.

关 键 词:5-氨基异喹啉酮 结肠癌 黏附作用 ICAM-1基因表达
文章编号:1000-9604(2007)02-0119-05
收稿时间:2007-01-15
修稿时间:2007-01-152007-04-02

Effect of 5-Aminoisoquinolinone on the adhesion of colon carcinoma
Wang Ya-lan,Hao Lan-xiang. Effect of 5-Aminoisoquinolinone on the adhesion of colon carcinoma[J]. Chinese Journal of Cancer Research, 2007, 19(2): 119-123. DOI: 10.1007/s11670-007-0119-0
Authors:Wang Ya-lan  Hao Lan-xiang
Affiliation:Department of Pathology, Chongqing Medical University, Chongqing 400016
Abstract:Objective 5-Aminoisoquinolinone, a water-soluble, potent inhibitor of the activity of poly (adenosine 5’-diphosphate ribose) polymerase, plays an important role in the tissue injury associated with ischaemia-reperfusion injury and inflammation by inhibiting the activity of poly (adenosine 5’-diphosphate ribose) polymerase and the expression of cell adhesion molecules such as ICAM-1, P-selectin et al. But how about it in the tumor is not clear. The aim of the present study was to study the effects of 5-Aminoisoquinolinon on the adhesion of colon carcinoma line HT-29 cells to human umbilical vein endothelial cells; and the effects of 5-Aminoisoquinolinon on the expression of ICAM-1, P-selectin and the activity of poly (adenosine 5’-diphosphate ribose) polymerase in colon carcinoma HT-29 cells. Methods The adhesion of HT-29 cells to human umbilical vein endothelial cells was detected by adhesive experiment. Immunocytochemically Streptavidin-Peroxidase method was used to investigate the expression of ICAM-1, P-selectin and Poly (adenosine 5’-diphosphate ribose)(the product of poly (adenosine 5’-diphosphate ribose) polymerase activation). Results the results of the adhesion assay of HT-29 cells to HUVEC showed that the OD570 value in each 5-AIQ-treated group was significant lower than that in the control group (5-AIQ-untreated) in a dose-dependent manner. The expression of ICAM-1, P-selectin and Poly (adenosine 5’-diphosphate ribose) was significant lower in 5-Aminoisoquinolinone-treated HT-29 cell group than that in 5-Aminoisoquinolinone-untreated groups. Conclusion The data suggest that 5-Aminoisoquinolinone can inhibit the adhesion of HT-29 cells to human umbilical vein endothelial cells. 5-Aminoisoquinolinone also can inhibit poly (adenosine 5’-diphosphate ribose) polymerase activation and the expressions of ICAM-1 and P-selectin in HT-29 cells. 5-Aminoisoquinolinone probably contributes to the prevention of tumor cell metastasis. Further study is needed. This work is supported by Natural Science Foundation Project of CQ CSTC (CSTC, 2006BB5288).
Keywords:5-Aminoisoquinolinone  Colon carcinoma  Adherence  ICAM-1 and P-selectin  Poly (adenosine 5'-diphosphate ribose) polymerase
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