肝癌及癌旁组织HBV DNA定点PCR研究

目的采用定点ＰＣＲ技术，研究ＨＢＶ的Ｓ和Ｃ基因在肝癌与癌旁组织的存在状态，探讨癌旁ＨＢｓＡｇ表达与Ｓ基因的关系。方法福尔马林固定，石蜡包埋肝癌及癌旁组织２８例，ＨＢｓＡｇ免疫组化后的切片分别切割癌组织、癌旁ＨＢｓＡｇ强阳性区（Ｐ２）及癌旁ＨＢｓＡｇ阴性区（Ｐ１）各１个低倍视野，作Ｓ及Ｃ基因ＰＣＲ。结果癌组织Ｓ基因较Ｃ基因检出率高（１８／２８ｖｓ１０／２８，Ｐ＜０．０５），癌旁二者无差异；癌旁中Ｐ１与Ｐ２的Ｓ及Ｃ基因检出率无显著性差异；高分化肝癌Ｓ和／或Ｃ基因检出率较中分化者低（２／５ｖｓ２０／２８，Ｐ＜０．０５）。结论癌组织中整合的ＨＢＶＤＮＡ为残缺不全的，ＨＢｓＡｇ强阳性与阴性区的Ｓ基因检出无显著性差异。

A study on HBVDNA in hepatocellular carcinoma and para_carcinomatous tissue by location PCR

AIMS\ To study S and C genes of HBVDNA in hepatocellular carcinoma(HCC) and para_HCC tissue by microdissection (location) PCR. METHODS\ Formalin fixed, paraffin embedded sections of 28 HCC, Para_HCC with strongly HBsAg( ) area (P 2) and HBsAg(-) area (P 1) in one low_power size tested by microdissection PCR of S and C genes. RESULTS\ Positivity rate of S gene was higher than that of C gene in HCC(P<0.05), while in para_HCC there were no significant difference between them. In para_HCC the positivity rates of S and C genes in P 1 and P 2 were similar. The positivity rates of S and/or C genes in highly differentiated HCC were lower than that of middle differentiated HCC (P<0.05). CONCLUSIONS\ The integrated HBVDNA is imcomplete in HCC tissue. The positivity rates of S gene in HBsAg strongly positive area and negative area were similar.